Journal article
Functions of CaBP1 and CaBP2 in the peripheral auditory system
Hearing research, Vol.364, pp.48-58
07/2018
DOI: 10.1016/j.heares.2018.04.001
PMID: 29661613
Abstract
CaBPs are a family of Ca2+ binding proteins related to calmodulin. Two CaBP family members, CaBP1 and CaBP2, are highly expressed in the cochlea. Here, we investigated the significance of CaBP1 and CaBP2 for hearing in mice lacking expression of these proteins (CaBP1 KO and CaBP2 KO) using auditory brain responses (ABRs) and distortion product otoacoustic emissions (DPOAEs). In CaBP1 KO mice, ABR wave I was larger in amplitude, and shorter in latency and faster in decay, suggestive of enhanced synchrony of auditory nerve fibers. This interpretation was supported by the greater excitability of CaBP1 KO than WT neurons in whole-cell patch clamp recordings of spiral ganglion neurons in culture, and normal presynaptic function of CaBP1 KO IHCs. DPOAEs and ABR thresholds were normal in 4-week old CaBP1 KO mice, but elevated ABR thresholds became evident at 32 kHz at 9 weeks, and at 8 and 16 kHz by 6 months of age. In contrast, CaBP2 KO mice exhibited significant ABR threshold elevations at 4 weeks of age that became more severe in the mid-frequency range by 9 weeks. Though normal at 4 weeks, DPOAEs in CaBP2 KO mice were significantly reduced in the mid-frequency range by 9 weeks. Our results reveal requirements for CaBP1 and CaBP2 in the peripheral auditory system and highlight the diverse modes by which CaBPs influence sensory processing.
•CaBP1 and CaBP2 are calmodulin-like proteins that are expressed in the cochlea.•CaBP1 regulates input resistance of spiral ganglion neurons and auditory nerve response properties.•CaBP2 regulates hearing sensitivity through functions in inner and outer hair cells.
Details
- Title: Subtitle
- Functions of CaBP1 and CaBP2 in the peripheral auditory system
- Creators
- Tian Yang - University of IowaNing Hu - Department of Otolaryngology Head-Neck Surgery, University of Iowa, Iowa City, IA 52242, USATina Pangršič - Synaptic Physiology of Mammalian Vestibular Hair Cells Group, Institute for Auditory Neuroscience and InnerEarLab, University Medical Center Göttingen, Göttingen, GermanySteven Green - Department of Otolaryngology Head-Neck Surgery, University of Iowa, Iowa City, IA 52242, USAMarlan Hansen - Department of Otolaryngology Head-Neck Surgery, University of Iowa, Iowa City, IA 52242, USAAmy Lee - Department of Molecular Physiology and Biophysics, University of Iowa, Iowa City, IA 52242, USA
- Resource Type
- Journal article
- Publication Details
- Hearing research, Vol.364, pp.48-58
- DOI
- 10.1016/j.heares.2018.04.001
- PMID
- 29661613
- NLM abbreviation
- Hear Res
- ISSN
- 0378-5955
- eISSN
- 1878-5891
- Publisher
- Elsevier B.V
- Grant note
- DOI: 10.13039/100000002, name: NIH, award: NS084190, DC009433; name: Iowa Center for Molecular Auditory Neuroscience, award: DC010362; DOI: 10.13039/100000005, name: U.S. Department of Defense, award: MR130438; DOI: 10.13039/501100001659, name: German Research Foundation, award: 1608, PA 2769/1-1; DOI: 10.13039/100001024, name: Roy J. Carver Charitable Trust
- Language
- English
- Date published
- 07/2018
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Neurology; Molecular Physiology and Biophysics; Biology; Neurosurgery; Otolaryngology
- Record Identifier
- 9983992042902771
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