Furcation Therapy With Enamel Matrix Derivative: Effects on the Subgingival Microbiome

Lucas A Queiroz; Renato C V Casarin; Shareef M Dabdoub; Dimitris N Tatakis; Enilson A Sallum; Purnima S Kumar

doi:10.1902/jop.2017.160542

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Furcation Therapy With Enamel Matrix Derivative: Effects on the Subgingival Microbiome

Journal article

Peer reviewed

Furcation Therapy With Enamel Matrix Derivative: Effects on the Subgingival Microbiome

Lucas A Queiroz, Renato C V Casarin, Shareef M Dabdoub, Dimitris N Tatakis, Enilson A Sallum and Purnima S Kumar

Journal of periodontology (1970), Vol.88(7), pp.617-625

07/2017

DOI: 10.1902/jop.2017.160542

PMID: 28304211

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Abstract

Although enamel matrix derivative (EMD) has been used to promote periodontal regeneration, little is known of its effect on the microbiome. Therefore, this investigation aims to identify changes in periodontal microbiome after treatment with EMD using a deep-sequencing approach. Thirty-nine patients with mandibular Class II buccal furcation defects were randomized to beta-tricalcium-phosphate/hydroxyapatite graft (BONE group), EMD+BONE, or EMD alone. Plaque was collected from furcation defects at baseline and 3 and 6 months post-treatment. Bacterial DNA was analyzed using terminal restriction fragment length polymorphism and 16S pyrotag sequencing, resulting in 169,000 classifiable sequences being compared with the Human Oral Microbiome Database. Statistical comparisons were made using parametric tests. At baseline, a total of 422 species were identified from the 39 defects, belonging to Fusobacterium, Pseudomonas, Streptococcus, Filifactor, and Parvimonas. All three regenerative procedures predictably altered the disease-associated microbiome, with a restitution of health-compatible species. However, EMD and BONE+EMD groups demonstrated more long-term reductions in a higher number of species than the BONE group (P <0.05), especially disease-associated species, e.g., Selenomonas noxia, F. alocis, and Fusobacterium. EMD treatment predictably alters a dysbiotic subgingival microbiome, decreasing pathogen richness and increasing commensal abundance. Further investigations are needed to investigate how this impacts regenerative outcomes.

Bone Substitutes - pharmacology

Bone Substitutes - therapeutic use

Dental Enamel Proteins - pharmacology

Dental Enamel Proteins - therapeutic use

Dental Plaque Index

DNA, Bacterial - isolation & purification

Female

Furcation Defects - microbiology

Furcation Defects - therapy

Gingiva - microbiology

Humans

Hydroxyapatites - pharmacology

Hydroxyapatites - therapeutic use

Male

Mandible - microbiology

Microbiota - drug effects

Middle Aged

Details

Title: Subtitle: Furcation Therapy With Enamel Matrix Derivative: Effects on the Subgingival Microbiome
Creators: Lucas A Queiroz - Universidade Estadual de Campinas (UNICAMP)
Renato C V Casarin - Universidade Estadual de Campinas (UNICAMP)
Shareef M Dabdoub - The Ohio State University
Dimitris N Tatakis - The Ohio State University
Enilson A Sallum - Universidade Estadual de Campinas (UNICAMP)
Purnima S Kumar - The Ohio State University
Resource Type: Journal article
Publication Details: Journal of periodontology (1970), Vol.88(7), pp.617-625
DOI: 10.1902/jop.2017.160542
PMID: 28304211
ISSN: 0022-3492
eISSN: 1943-3670
Grant note: R01 DE022579 / NIDCR NIH HHS T32 DE014320 / NIDCR NIH HHS
Language: English
Date published: 07/2017
Academic Unit: Dental Research; Periodontics
Record Identifier: 9984367631802771

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