Further standardization of broth microdilution methodology for in vitro susceptibility testing of caspofungin against Candida species by use of an international collection of more than 3,000 clinical isolates

M A Pfaller; S A Messer; L Boyken; C Rice; S Tendolkar; R J Hollis; D J Diekema

doi:10.1128/JCM.42.7.3117-3119.2004

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Further standardization of broth microdilution methodology for in vitro susceptibility testing of caspofungin against Candida species by use of an international collection of more than 3,000 clinical isolates

Journal article

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Further standardization of broth microdilution methodology for in vitro susceptibility testing of caspofungin against Candida species by use of an international collection of more than 3,000 clinical isolates

M A Pfaller, S A Messer, L Boyken, C Rice, S Tendolkar, R J Hollis and D J Diekema

Journal of clinical microbiology, Vol.42(7), pp.3117-3119

07/2004

DOI: 10.1128/JCM.42.7.3117-3119.2004

PMCID: PMC446304

PMID: 15243069

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Abstract

The influence of test variables on in vitro susceptibility testing of caspofungin was examined with 694 isolates of Candida albicans including seven laboratory-derived glucan synthesis mutants. The conditions providing the greatest separation between the mutant strains and the clinical isolates were RPMI medium, MIC end point criterion of partial inhibition, and incubation for 24 h. These testing conditions were then applied to 3,322 isolates of Candida spp. (3,314 clinical isolates and eight glucan synthesis mutants). Among the 11 isolates for which caspofungin MICs were >/=2 microg/ml, eight were accounted for by the glucan synthesis mutants. The MICs for >99% of isolates were </=1 microg/ml, and thus these isolates were differentiated from strains with reduced in vitro and in vivo susceptibilities to caspofungin.

Antifungal Agents - pharmacology

Peptides, Cyclic

Microbial Sensitivity Tests - standards

Humans

Echinocandins

Candida - drug effects

Lipopeptides

Peptides - pharmacology

Quality Control

Details

Title: Subtitle: Further standardization of broth microdilution methodology for in vitro susceptibility testing of caspofungin against Candida species by use of an international collection of more than 3,000 clinical isolates
Creators: M A Pfaller - Medical Microbiology Division, C606 GH, Department of Pathology, University of Iowa College of Medicine, Iowa City, IA 52242, USA. michael-pfaller@uiowa.edu
S A Messer
L Boyken
C Rice
S Tendolkar
R J Hollis
D J Diekema
Resource Type: Journal article
Publication Details: Journal of clinical microbiology, Vol.42(7), pp.3117-3119
DOI: 10.1128/JCM.42.7.3117-3119.2004
PMID: 15243069
PMCID: PMC446304
NLM abbreviation: J Clin Microbiol
ISSN: 0095-1137
eISSN: 1098-660X
Publisher: United States
Language: English
Date published: 07/2004
Academic Unit: Infectious Diseases; Epidemiology; Pathology; Internal Medicine
Record Identifier: 9983986371402771

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