Fyn binds to and phosphorylates T cell immunoglobulin and mucin domain-1 (Tim-1)

Miranda L Curtiss; Bruce S Hostager; Elizabeth Stepniak; Melody Singh; Natalie Manhica; Judit Knisz; Geri Traver; Paul D Rennert; John D Colgan; Paul B Rothman

doi:10.1016/j.molimm.2011.03.023

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Fyn binds to and phosphorylates T cell immunoglobulin and mucin domain-1 (Tim-1)

Journal article

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Peer reviewed

Fyn binds to and phosphorylates T cell immunoglobulin and mucin domain-1 (Tim-1)

Miranda L Curtiss, Bruce S Hostager, Elizabeth Stepniak, Melody Singh, Natalie Manhica, Judit Knisz, Geri Traver, Paul D Rennert, John D Colgan and Paul B Rothman

Molecular immunology, Vol.48(12), pp.1424-1431

2011

DOI: 10.1016/j.molimm.2011.03.023

PMCID: PMC3109159

PMID: 21513984

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https://www.ncbi.nlm.nih.gov/pmc/articles/3109159View

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Abstract

The gene encoding T cell immunoglobulin and mucin domain-1 (Tim-1) is linked to atopy and asthma susceptibility in mice and humans. Tim-1 is a transmembrane protein expressed on activated lymphocytes and appears to have a role as a co-stimulatory receptor in T cells. The protein has not been shown to have enzymatic activity but contains a site within its cytoplasmic tail predicted to be a target for tyrosine kinases. Here, we show that Tim-1 can associate with the kinase Fyn, a member of the Src family of tyrosine kinases. This association does not require Fyn's kinase activity and is independent of the phosphorylation of a conserved tyrosine present within the cytoplasmic tail of Tim-1. Fyn is necessary for phosphorylation of this tyrosine in Tim-1 and the phosphorylation of Tim-1 varies with the levels of Fyn present in cells. These data suggest a role for Fyn in the signaling downstream of Tim-1.

B cell

Signaling

Fyn

Havcr-1

Tim-1

Details

Title: Subtitle: Fyn binds to and phosphorylates T cell immunoglobulin and mucin domain-1 (Tim-1)
Creators: Miranda L Curtiss - Department of Internal Medicine, University of Iowa Carver College of Medicine. 200 Hawkins Drive, Iowa City, IA 52242, USA
Bruce S Hostager - Department of Internal Medicine, University of Iowa Carver College of Medicine. 200 Hawkins Drive, Iowa City, IA 52242, USA
Elizabeth Stepniak - Department of Internal Medicine, University of Iowa Carver College of Medicine. 200 Hawkins Drive, Iowa City, IA 52242, USA
Melody Singh - Department of Internal Medicine, University of Iowa Carver College of Medicine. 200 Hawkins Drive, Iowa City, IA 52242, USA
Natalie Manhica - Department of Internal Medicine, University of Iowa Carver College of Medicine. 200 Hawkins Drive, Iowa City, IA 52242, USA
Judit Knisz - Department of Internal Medicine, University of Iowa Carver College of Medicine. 200 Hawkins Drive, Iowa City, IA 52242, USA
Geri Traver - Department of Internal Medicine, University of Iowa Carver College of Medicine. 200 Hawkins Drive, Iowa City, IA 52242, USA
Paul D Rennert - Biogen Idec, 12 Cambridge Center, Cambridge, MA 01746, USA
John D Colgan - Department of Internal Medicine, University of Iowa Carver College of Medicine. 200 Hawkins Drive, Iowa City, IA 52242, USA
Paul B Rothman - Department of Internal Medicine, University of Iowa Carver College of Medicine. 200 Hawkins Drive, Iowa City, IA 52242, USA
Resource Type: Journal article
Publication Details: Molecular immunology, Vol.48(12), pp.1424-1431
DOI: 10.1016/j.molimm.2011.03.023
PMID: 21513984
PMCID: PMC3109159
NLM abbreviation: Mol Immunol
ISSN: 0161-5890
eISSN: 1872-9142
Publisher: Elsevier Ltd
Language: English
Date published: 2011
Academic Unit: Microbiology and Immunology; Anatomy and Cell Biology; Immunology; Internal Medicine
Record Identifier: 9984025578102771

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