Journal article
Gα12/13 regulate epiboly by inhibiting E-cadherin activity and modulating the actin cytoskeleton
The Journal of cell biology, Vol.184(6), pp.909-921
03/23/2009
DOI: 10.1083/jcb.200805148
PMCID: PMC2664974
PMID: 19307601
Abstract
Epiboly spreads and thins the blastoderm over the yolk cell during zebrafish gastrulation, and involves coordinated movements of several cell layers. Although recent studies have begun to elucidate the processes that underlie these epibolic movements, the cellular and molecular mechanisms involved remain to be fully defined. Here, we show that gastrulae with altered Gα12/13 signaling display delayed epibolic movement of the deep cells, abnormal movement of dorsal forerunner cells, and dissociation of cells from the blastoderm, phenocopying e-cadherin mutants. Biochemical and genetic studies indicate that Gα12/13 regulate epiboly, in part by associating with the cytoplasmic terminus of E-cadherin, and thereby inhibiting E-cadherin activity and cell adhesion. Furthermore, we demonstrate that Gα12/13 modulate epibolic movements of the enveloping layer by regulating actin cytoskeleton organization through a RhoGEF/Rho-dependent pathway. These results provide the first in vivo evidence that Gα12/13 regulate epiboly through two distinct mechanisms: limiting E-cadherin activity and modulating the organization of the actin cytoskeleton.
Details
- Title: Subtitle
- Gα12/13 regulate epiboly by inhibiting E-cadherin activity and modulating the actin cytoskeleton
- Creators
- Fang Lin - Department of Pharmacology, Vanderbilt University Medical Center, Nashville, TN 37232, Department of Pharmacology and Department of Anatomy and Cell Biology, Carver College of Medicine, University of Iowa, Iowa City, IA 52242Songhai Chen - Department of Pharmacology and Department of Anatomy and Cell Biology, Carver College of Medicine, University of Iowa, Iowa City, IA 52242Diane S Sepich - Department of Biological Sciences, Vanderbilt University, Nashville, TN 37235Jennifer Ray Panizzi - Department of Biological Sciences, Vanderbilt University, Nashville, TN 37235Sherry G Clendenon - Department of Medicine, Indiana University Medical Center, Indianapolis, IN 46202James A Marrs - Department of Medicine, Indiana University Medical Center, Indianapolis, IN 46202Heidi E Hamm - Department of Pharmacology, Vanderbilt University Medical Center, Nashville, TN 37232Lilianna Solnica-Krezel - Department of Biological Sciences, Vanderbilt University, Nashville, TN 37235
- Resource Type
- Journal article
- Publication Details
- The Journal of cell biology, Vol.184(6), pp.909-921
- DOI
- 10.1083/jcb.200805148
- PMID
- 19307601
- PMCID
- PMC2664974
- NLM abbreviation
- J Cell Biol
- ISSN
- 0021-9525
- eISSN
- 1540-8140
- Language
- English
- Date published
- 03/23/2009
- Academic Unit
- Anatomy and Cell Biology; Iowa Neuroscience Institute; Craniofacial Anomalies Research Center; Fraternal Order of Eagles Diabetes Research Center; Neuroscience and Pharmacology; Internal Medicine
- Record Identifier
- 9984040594602771
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