Journal article
G-protein-coupled estrogen receptor 1 is anatomically positioned to modulate synaptic plasticity in the mouse hippocampus
The Journal of neuroscience, Vol.35(6), pp.2384-2397
02/11/2015
DOI: 10.1523/JNEUROSCI.1298-14.2015
PMCID: PMC4323523
PMID: 25673833
Abstract
Both estrous cycle and sex affect the numbers and types of neuronal and glial profiles containing the classical estrogen receptors α and β, and synaptic levels in the rodent dorsal hippocampus. Here, we examined whether the membrane estrogen receptor, G-protein-coupled estrogen receptor 1 (GPER1), is anatomically positioned in the dorsal hippocampus of mice to regulate synaptic plasticity. By light microscopy, GPER1-immunoreactivity (IR) was most noticeable in the pyramidal cell layer and interspersed interneurons, especially those in the hilus of the dentate gyrus. Diffuse GPER1-IR was found in all lamina but was most dense in stratum lucidum of CA3. Ultrastructural analysis revealed discrete extranuclear GPER1-IR affiliated with the plasma membrane and endoplasmic reticulum of neuronal perikarya and dendritic shafts, synaptic specializations in dendritic spines, and clusters of vesicles in axon terminals. Moreover, GPER1-IR was found in unmyelinated axons and glial profiles. Overall, the types and amounts of GPER1-labeled profiles were similar between males and females; however, in females elevated estrogen levels generally increased axonal labeling. Some estradiol-induced changes observed in previous studies were replicated by the GPER agonist G1: G1 increased PSD95-IR in strata oriens, lucidum, and radiatum of CA3 in ovariectomized mice 6 h after administration. In contrast, estradiol but not G1 increased Akt phosphorylation levels. Instead, GPER1 actions in the synapse may be due to interactions with synaptic scaffolding proteins, such as SAP97. These results suggest that although estrogen's actions via GPER1 may converge on the same synaptic elements, different pathways are used to achieve these actions.
Details
- Title: Subtitle
- G-protein-coupled estrogen receptor 1 is anatomically positioned to modulate synaptic plasticity in the mouse hippocampus
- Creators
- Elizabeth M Waters - Harold and Margaret Milliken Hatch Laboratory of Neuroendocrinology, The Rockefeller University, New York, New York 10065, ewaters@rockfeller.eduLouisa I Thompson - Harold and Margaret Milliken Hatch Laboratory of Neuroendocrinology, The Rockefeller University, New York, New York 10065, Brain and Mind Research Institute, Weill Cornell Medical College, New York, New York 10065Parth Patel - Brain and Mind Research Institute, Weill Cornell Medical College, New York, New York 10065Andreina D Gonzales - Brain and Mind Research Institute, Weill Cornell Medical College, New York, New York 10065Hector Zhiyu Ye - Brain and Mind Research Institute, Weill Cornell Medical College, New York, New York 10065Edward J Filardo - Department of Medicine, Brown University, Providence, Rhode Island 02903, andDeborah J Clegg - Department of Internal Medicine, University of Texas Southwestern Medical Center at Dallas, Dallas, Texas 75390Jolanta Gorecka - Harold and Margaret Milliken Hatch Laboratory of Neuroendocrinology, The Rockefeller University, New York, New York 10065Keith T Akama - Harold and Margaret Milliken Hatch Laboratory of Neuroendocrinology, The Rockefeller University, New York, New York 10065Bruce S McEwen - Harold and Margaret Milliken Hatch Laboratory of Neuroendocrinology, The Rockefeller University, New York, New York 10065Teresa A Milner - Harold and Margaret Milliken Hatch Laboratory of Neuroendocrinology, The Rockefeller University, New York, New York 10065, Brain and Mind Research Institute, Weill Cornell Medical College, New York, New York 10065
- Resource Type
- Journal article
- Publication Details
- The Journal of neuroscience, Vol.35(6), pp.2384-2397
- Publisher
- United States
- DOI
- 10.1523/JNEUROSCI.1298-14.2015
- PMID
- 25673833
- PMCID
- PMC4323523
- ISSN
- 0270-6474
- eISSN
- 1529-2401
- Grant note
- AG039850 / NIA NIH HHS NS007080 / NINDS NIH HHS DK088761 / NIDDK NIH HHS DA08259 / NIDA NIH HHS HL096571 / NHLBI NIH HHS AG16765 / NIA NIH HHS P01 HL096571 / NHLBI NIH HHS R21 AG039850 / NIA NIH HHS R01 DA008259 / NIDA NIH HHS T32 DK007313 / NIDDK NIH HHS P01 AG016765 / NIA NIH HHS R01 NS007080 / NINDS NIH HHS P01 DK088761 / NIDDK NIH HHS R01 HL098351 / NHLBI NIH HHS HL098351 / NHLBI NIH HHS DK07313 / NIDDK NIH HHS
- Language
- English
- Date published
- 02/11/2015
- Academic Unit
- Pharmaceutical Sciences and Experimental Therapeutics; Surgery; Internal Medicine
- Record Identifier
- 9984051796802771
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