Journal article
G1 shortening following unbalanced growth : a specific v. nonspecific effect
Cell proliferation, Vol.25(3), pp.251-260
1992
DOI: 10.1111/j.1365-2184.1992.tb01400.x
PMID: 1596538
Abstract
The synthesis and abundance of proteins were examined in synchronous populations of HeLa cells under conditions in which the lengthening of S phase, by inhibiting DNA synthesis, resulted in shortening of G1 in the subsequent generation. Mitotically collected cells were resynchronized by incubation with 3 PM aphidicolin from 3 to 12 h after mitotic selection; they were blocked again at various times thereafter to induce unbalanced growth. Cells were labelled with [35S]‐methionine before and after release from the block to study the changes in protein synthesis. Triton X‐100 soluble and insoluble proteins were analysed by 7–15% gradient SDS‐PAGE, and radioactivity incorporation was quantified by liquid‐scintillation counting. The degree of G1 shortening correlated with S phase position, increasing gradually from early S and reaching maximum when cells were blocked half‐way through S phase. Synthesis of proteins of 120, 66, and 51 kDa was stimulated, and synthesis of a new protein of 57kDa was observed, in cells in which DNA synthesis had been blocked in mid‐S. These proteins also showed increased accumulation. These results suggest that the shortening of GI, induced by the prior arrest of cell‐cycle progression, is associated with synthesis of specific proteins rather than the non‐specific accumulation of cellular proteins through unbalanced growth.
Details
- Title: Subtitle
- G1 shortening following unbalanced growth : a specific v. nonspecific effect
- Creators
- P. C GOSWAMI - Washington univ. medical cent., radiation oncology cent., stn. cancer biology, St Louis, United StatesR HIGASHIKUBO - Washington univ. medical cent., radiation oncology cent., stn. cancer biology, St Louis, United StatesL. T TOLMACH - Washington univ. medical cent., radiation oncology cent., stn. cancer biology, St Louis, United StatesJ. L ROTI ROTI - Washington univ. medical cent., radiation oncology cent., stn. cancer biology, St Louis, United States
- Resource Type
- Journal article
- Publication Details
- Cell proliferation, Vol.25(3), pp.251-260
- DOI
- 10.1111/j.1365-2184.1992.tb01400.x
- PMID
- 1596538
- NLM abbreviation
- Cell Prolif
- ISSN
- 0960-7722
- eISSN
- 1365-2184
- Publisher
- Blackwell; Oxford
- Language
- English
- Date published
- 1992
- Academic Unit
- Radiation Oncology
- Record Identifier
- 9984047641702771
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