GAPDH is critical for superior efficacy of female bone marrow-derived mesenchymal stem cells on pulmonary hypertension

Rubin Tan; Jiansha Li; Xiaochun Peng; Liping Zhu; Lei Cai; Tao Wang; Yuan Su; Kaikobad Irani; Qinghua Hu

doi:10.1093/cvr/cvt165

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GAPDH is critical for superior efficacy of female bone marrow-derived mesenchymal stem cells on pulmonary hypertension

Journal article

Open access

Peer reviewed

GAPDH is critical for superior efficacy of female bone marrow-derived mesenchymal stem cells on pulmonary hypertension

Rubin Tan, Jiansha Li, Xiaochun Peng, Liping Zhu, Lei Cai, Tao Wang, Yuan Su, Kaikobad Irani and Qinghua Hu

Cardiovascular research, Vol.100(1), pp.19-27

10/01/2013

DOI: 10.1093/cvr/cvt165

PMID: 23801767

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Abstract

Pulmonary arterial hypertension, a chronic lung disease, remains an unacceptable prognosis despite significant advances in conventional therapies. Stem cell therapy represents a novel and effective modality. This study was aimed to add new insight in gender differences of bone marrow-derived mesenchymal stem cells on therapy against pulmonary arterial hypertension and the underlying mechanism. By in vivo experiments, we showed for the first time female bone marrow-derived mesenchymal stem cells possessed a better therapeutic potential against monocrotaline-induced pulmonary arterial hypertension in C57BL/6J mice compared with male counterparts. In vitro experiments demonstrated superior function of female bone marrow-derived mesenchymal stem cells in cell proliferation, migration and [Ca(2+)]i kinetics. Moreover, we unexpectedly found that, compared with male ones, female bone marrow-derived mesenchymal stem cells had a higher expression level of glyceraldehyde-3-phosphate dehydrogenase and manipulations of its expression in female or male bone marrow-derived mesenchymal stem cells profoundly affected their cellular behaviours and therapeutic efficacies against pulmonary arterial hypertension. Our results suggest that glyceraldehyde-3-phosphate dehydrogenase plays a critical role in determining the superior functions of female bone marrow-derived mesenchymal stem cells in cell therapy against pulmonary arterial hypertension by regulating [Ca(2+)]i signal-associated cellular behaviours.

Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating) - physiology

Bone Marrow Cells - enzymology

Calcium - metabolism

Mice, Inbred C57BL

Cells, Cultured

Familial Primary Pulmonary Hypertension

Male

Sex Characteristics

Hypertension, Pulmonary - therapy

Animals

Female

Mice

Mesenchymal Stem Cell Transplantation

Mesenchymal Stromal Cells - physiology

Details

Title: Subtitle: GAPDH is critical for superior efficacy of female bone marrow-derived mesenchymal stem cells on pulmonary hypertension
Creators: Rubin Tan - Huazhong University of Science and Technology
Jiansha Li
Xiaochun Peng
Liping Zhu
Lei Cai - Huazhong University of Science and Technology
Tao Wang
Yuan Su
Kaikobad Irani
Qinghua Hu
Resource Type: Journal article
Publication Details: Cardiovascular research, Vol.100(1), pp.19-27
DOI: 10.1093/cvr/cvt165
PMID: 23801767
NLM abbreviation: Cardiovasc Res
ISSN: 0008-6363
eISSN: 1755-3245
Publisher: England
Language: English
Date published: 10/01/2013
Academic Unit: Cardiovascular Medicine; Radiation Oncology; Fraternal Order of Eagles Diabetes Research Center; Internal Medicine
Record Identifier: 9984047667602771

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