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GATA‐3 is expressed in association with estrogen receptor in breast cancer
Journal article   Peer reviewed

GATA‐3 is expressed in association with estrogen receptor in breast cancer

Renee V. Hoch, Devon A. Thompson, Robin J. Baker and Ronald J. Weigel
International journal of cancer, Vol.84(2), pp.122-128
04/20/1999
DOI: 10.1002/(SICI)1097-0215(19990420)84:2<122::AID-IJC5>3.0.CO;2-S

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Abstract

To better understand the molecular basis for the hormone‐responsive phenotype in breast cancer, we have used a human cDNA array to compare patterns of gene expression between breast carcinoma cell lines discordant for estrogen receptor (ER) expression. These experiments indicated abundant expression of the transcription factor GATA‐3 in the ER‐positive cell lines MCF7 and T‐47D, with minimal or no expression in the ER‐negative cells lines MDA‐MB‐231 and HBL‐100. Northern blot analysis of a panel of human breast carcinoma cell lines demonstrated a correlation between ER and GATA‐3 expression. Studies of MCF7 cells grown in the absence or presence β‐estradiol indicated that GATA‐3 expression was not responsive to estradiol. Protein immunoprecipitation and gel shift analysis confirmed the presence of functional GATA‐3 protein in MCF7 but not in HBL‐100 nuclear extracts. A panel of 47 primary breast cancers was characterized for expression of ER and GATA‐3 using immunoperoxidase assay. In primary tumors, a statistically significant correlation between ER and GATA‐3 expression was established (p < 0.0001, χ2). Our results indicate that GATA‐3, in association with ER, is likely to regulate genes critical to the hormone‐responsive breast cancer phenotype. Int. J. Cancer (Pred. Oncol.) 84:122–128, 1999. © 1999 Wiley‐Liss, Inc.

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