Journal article
GFP expressing locus coeruleus neurons obtained from Prp57 transgenic mice exhibit intrinsic CO2/H+ responses in primary cell culture
The FASEB journal, Vol.21(6), pp.LB121-LB121
2007
DOI: 10.1096/fasebj.21.6.LB121-c
Abstract
CO2/H+ central chemoreceptors sense fluctuations in pH and modulate the respiratory network to adjust ventilation. Neurons excised from Prp57 transgenic mice expressing green fluorescent protein (GFP) in the locus coeruleus (LC) were digested, triturated, and plated onto coverslips. We hypothesized that LC neurons would exhibit intrinsic CO2/H+ responses in primary culture. Quantifiable changes in firing rate (FR) were obtained using perforated patch clamp recordings after pharmacological blockade of fast inhibitory and excitatory transmission. FR was assessed under control conditions (5% CO2; ~pH 7.4) and during respiratory acidosis (9% CO2; ~pH 7.2) and respiratory alkalosis (3% CO2; ~pH 7.6). Steady state FR was calculated and expressed as a percent of control. Acidosis stimulated neurons exhibited a reproducible increase in FR as pH decreased—ranging from 120 to >250% of control. Stimulated neurons exhibited a reduction in FR during alkalosis. GFP‐LC neurons exhibit a differential and intrinsic response to changes in CO2/H+ in primary culture. In conclusion, Prp57 GFP‐LC neurons may serve as a cellular model for evalu1ating central chemosensory mechanisms.
Funded by NS047422 to SMJ.
Details
- Title: Subtitle
- GFP expressing locus coeruleus neurons obtained from Prp57 transgenic mice exhibit intrinsic CO2/H+ responses in primary cell culture
- Creators
- Sheree M Johnson - Yale University School of MedicineMusa A Haxhiu - Case Western Reserve UniversityGeorge B Richerson - Yale University School of Medicine
- Resource Type
- Journal article
- Publication Details
- The FASEB journal, Vol.21(6), pp.LB121-LB121
- Publisher
- The Federation of American Societies for Experimental Biology
- DOI
- 10.1096/fasebj.21.6.LB121-c
- ISSN
- 0892-6638
- eISSN
- 1530-6860
- Number of pages
- 1
- Language
- English
- Date published
- 2007
- Academic Unit
- Neurology; Molecular Physiology and Biophysics; Iowa Neuroscience Institute; Neurosurgery
- Record Identifier
- 9984071951702771
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