Journal article
GGA proteins bind ubiquitin to facilitate sorting at the trans -Golgi network
Nature cell biology, Vol.6(3), pp.252-259
03/2004
DOI: 10.1038/ncb1107
PMID: 15039776
Abstract
Ubiquitination functions as a sorting signal for lysosomal degradation of cell-surface proteins by facilitating their internalization from the plasma membrane and incorporation into lumenal vesicles of multivesicular bodies (MVBs). Ubiquitin may also mediate sorting of proteins from the trans-Golgi network (TGN) to the endosome, thereby preventing their appearance on the cell surface and hastening their degradation in the lysosome-vacuole. Substantiation of a direct ubiquitin-dependent TGN sorting pathway relies in part on identifying candidate machinery that may function as a ubiquitin-sorting 'receptor'at the TGN. Members of the GGA family of coat proteins localize to the TGN and promote the incorporation of proteins into clathrin-coated vesicles destined for transport to endosomes. We show that the GGA coat proteins bind directly to ubiquitin through their GAT domain and demonstrate that this interaction is required for the ubiquitin-dependent sorting of the Gap1 amino acid transporter from the TGN to endosomes. Thus, GGA proteins fulfill the role of ubiquitin sorting receptors at the TGN.
Details
- Title: Subtitle
- GGA proteins bind ubiquitin to facilitate sorting at the trans -Golgi network
- Creators
- Patricia M Scott - University of Minnesota, DuluthRobert C Piper - University of IowaPatricia S Bilodeau - University of IowaOlga Zhdankina - University of Minnesota, DuluthStanley C Winistorfer - University of IowaMelissa J Hauglund - University of Minnesota, DuluthMargaret M Allaman - University of IowaWilliam R Kearney - University of IowaAndrew D Robertson - University of IowaAnnette L Boman - University of Minnesota, Duluth
- Resource Type
- Journal article
- Publication Details
- Nature cell biology, Vol.6(3), pp.252-259
- DOI
- 10.1038/ncb1107
- PMID
- 15039776
- ISSN
- 1465-7392
- eISSN
- 1476-4679
- Language
- English
- Date published
- 03/2004
- Academic Unit
- Radiology; Molecular Physiology and Biophysics; Medicine Administration; Internal Medicine
- Record Identifier
- 9984297499402771
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