GIPC Binds to the Human Lutropin Receptor (hLHR) through an Unusual PDZ Domain Binding Motif, and It Regulates the Sorting of the Internalized Human Choriogonadotropin and the Density of Cell Surface hLHR

Takashi Hirakawa; Colette Galet; Mikiko Kishi; Mario Ascoli

doi:10.1074/jbc.M306557200

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GIPC Binds to the Human Lutropin Receptor (hLHR) through an Unusual PDZ Domain Binding Motif, and It Regulates the Sorting of the Internalized Human Choriogonadotropin and the Density of Cell Surface hLHR

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GIPC Binds to the Human Lutropin Receptor (hLHR) through an Unusual PDZ Domain Binding Motif, and It Regulates the Sorting of the Internalized Human Choriogonadotropin and the Density of Cell Surface hLHR

Takashi Hirakawa, Colette Galet, Mikiko Kishi and Mario Ascoli

The Journal of biological chemistry, Vol.278(49), pp.49348-49357

12/05/2003

DOI: 10.1074/jbc.M306557200

PMID: 14507927

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Abstract

By using a yeast two-hybrid screen we identified GIPC (GAIP-interacting protein C terminus), a protein with a type I PDZ domain as a novel human lutropin receptor (hLHR) binding partner. Pull-down and immunoprecipitation assays confirmed this interaction and showed that it is dependent on the PDZ domain of GIPC and the C-terminal tetrapeptide of the hLHR. To characterize the functional consequences of the GIPC-hLHR interaction, we used a small interfering RNA against GIPC to generate a clonal cell line that is deficient in GIPC. Studies with this cell line reveal that GIPC is partially responsible for the recycling of the hormone that is internalized by the hLHR and also for maintaining a relatively constant level of hLHR at the cell surface during hormone internalization.

Details

Title: Subtitle: GIPC Binds to the Human Lutropin Receptor (hLHR) through an Unusual PDZ Domain Binding Motif, and It Regulates the Sorting of the Internalized Human Choriogonadotropin and the Density of Cell Surface hLHR
Creators: Takashi Hirakawa - University of Iowa
Colette Galet - University of Iowa
Mikiko Kishi - University of Iowa
Mario Ascoli - University of Iowa
Resource Type: Journal article
Publication Details: The Journal of biological chemistry, Vol.278(49), pp.49348-49357
DOI: 10.1074/jbc.M306557200
PMID: 14507927
NLM abbreviation: J Biol Chem
ISSN: 0021-9258
eISSN: 1083-351X
Publisher: Elsevier Inc
Number of pages: 10
Language: English
Date published: 12/05/2003
Academic Unit: Injury Prevention Research Center; Neuroscience and Pharmacology; University of Iowa Health Care
Record Identifier: 9985137927902771

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