Journal article
GL7 ligand expression defines a novel subset of CD4+TRM cells in lungs recovered from pneumococcus
Mucosal immunology, Vol.16(5), pp.699-710
10/01/2023
DOI: 10.1016/j.mucimm.2023.07.004
PMCID: PMC10591822
PMID: 37604254
Abstract
Streptococcus pneumoniae is the most common etiology of bacterial pneumonia, one of the leading causes of death in children and the elderly worldwide. During non-lethal infections with S. pneumoniae, lymphocytes accumulate in the lungs and protect against reinfection with serotype-mismatched strains. Cluster of differentiation CD4+ resident memory T (TRM) cells are known to be crucial for this protection, but the diversity of lung CD4+ TRM cells has yet to be fully delineated. We aimed to identify unique subsets and their contributions to lung immunity. After recovery from pneumococcal infections, we identified a distinct subset of CD4+ T cells defined by the phenotype CD11ahiCD69+GL7+ in mouse lungs. Phenotypic analyses for markers of lymphocyte memory and residence demonstrated that GL7+ T cells are a subset of CD4+ TRM cells. Functional studies revealed that unlike GL7- TRM subsets that were mostly (RAR-related Orphan Receptor gamma T) ROR gamma T+, GL7+ TRM cells exhibited higher levels of (T-box expressed in T cells) T-bet and Gata-3, corresponding with increased synthesis of interferon-gamma, interleukin-13, and interleukin-5, inherent to both T helper 1 (TH1) and TH2 functions. Thus, we propose that these cells provide novel contributions during pneumococcal pneumonia, serving as important determinants of lung immunity.
Details
- Title: Subtitle
- GL7 ligand expression defines a novel subset of CD4+TRM cells in lungs recovered from pneumococcus
- Creators
- Carolina Lyon De Ana - Boston UniversityAnukul T. Shenoy - Boston UniversityKimberly A. Barker - Boston UniversityEmad I. Arafa - Boston UniversityNeelou S. Etesami - Boston UniversityFiliz T. Korkmaz - Boston UniversityAlicia M. Soucy - Boston UniversityMichael P. Breen - Boston UniversityIan M. C. Martin - Boston UniversityBrian R. Tilton - Boston UniversityPriyadharshini Devarajan - University of Massachusetts Chan Medical SchoolNicholas A. Crossland - Boston UniversityRiley M. F. Pihl - Boston UniversityWesley N. Goltry - Boston UniversityAnna C. Belkina - Boston UniversityMatthew R. Jones - Boston UniversityLee J. Quinton - Boston UniversityJoseph P. Mizgerd - Boston University
- Resource Type
- Journal article
- Publication Details
- Mucosal immunology, Vol.16(5), pp.699-710
- Publisher
- Elsevier
- DOI
- 10.1016/j.mucimm.2023.07.004
- PMID
- 37604254
- PMCID
- PMC10591822
- ISSN
- 1933-0219
- eISSN
- 1935-3456
- Number of pages
- 12
- Grant note
- K99 HL157555; F31 HL142199; F31 HL147397; F30 HL158109; F32 HL147461; S10-OD026983; SS10-OD030269; RO1 HL136725; RO1 GM120060; RO1 HL111449; RO1 AI115053; R35 HL135756; R33 HL137081; T32 HL007035 / NIH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA
- Language
- English
- Date published
- 10/01/2023
- Academic Unit
- Microbiology and Immunology
- Record Identifier
- 9984696651102771
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