GLI2 promoter hypermethylation in saliva of children with a respiratory allergy

Sabine A S Langie; Matthieu Moisse; Katarzyna Szarc Vel Szic; Ellen Van Der Plas; Gudrun Koppen; Sofie De Prins; Tijs Louwies; Vera Nelen; Guy Van Camp; Diether Lambrechts; Greet Schoeters; Wim Vanden Berghe; Patrick De Boever

doi:10.1186/s13148-018-0484-1

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GLI2 promoter hypermethylation in saliva of children with a respiratory allergy

Journal article

Open access

Peer reviewed

GLI2 promoter hypermethylation in saliva of children with a respiratory allergy

Sabine A S Langie, Matthieu Moisse, Katarzyna Szarc Vel Szic, Ellen Van Der Plas, Gudrun Koppen, Sofie De Prins, Tijs Louwies, Vera Nelen, Guy Van Camp, Diether Lambrechts, …

Clinical epigenetics, Vol.10(1), pp.50-50

2018

DOI: 10.1186/s13148-018-0484-1

PMCID: PMC5896137

PMID: 29682088

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Abstract

The prevalence of respiratory allergy in children is increasing. Epigenetic DNA methylation changes are plausible underlying molecular mechanisms. Saliva samples collected in substudies of two longitudinal birth cohorts in Belgium (FLEHS1 & FLEHS2) were used to discover and confirm DNA methylation signatures that can differentiate individuals with respiratory allergy from healthy subjects. Genome-wide analysis with Illumina Methylation 450K BeadChips revealed 23 differentially methylated gene regions (DMRs) in saliva from 11y old allergic children (N=26) vs. controls (N=20) in FLEHS1. A subset of 7 DMRs was selected for confirmation by iPLEX MassArray analysis. First, iPLEX analysis was performed in the same 46 FLEHS1 samples for analytical confirmation of the findings obtained during the discovery phase. iPLEX results correlated significantly with the 450K array data ( <0.0001) and confirmed 4 out of the 7 DMRs. Aiming for additional biological confirmation, the 7 DMRs were analyzed using iPLEX in a substudy of an independent birth cohort (FLEHS2; N=19 cases vs. 20 controls, aged 5 years). One DMR in the promoter region showed a consistent statistically significant hypermethylation in individuals with respiratory allergy across the two birth cohorts and technologies. In addition to its involvement in TGF-β signaling and T-helper differentiation, has a regulating role in lung development. is considered an interesting candidate DNA methylation marker for respiratory allergy.

Belgium

Case-Control Studies

Child

Child, Preschool

CpG Islands

DNA Methylation

Epigenesis, Genetic

Female

Genome-Wide Association Study

Humans

Longitudinal Studies

Male

Nuclear Proteins - genetics

Promoter Regions, Genetic

Respiratory Hypersensitivity - genetics

Saliva - chemistry

Zinc Finger Protein Gli2 - genetics

Details

Title: Subtitle: GLI2 promoter hypermethylation in saliva of children with a respiratory allergy
Creators: Sabine A S Langie - Hasselt University
Matthieu Moisse - KU Leuven
Katarzyna Szarc Vel Szic - University of Antwerp
Ellen Van Der Plas - University of Antwerp
Gudrun Koppen - VITO- Sustainable Health, Mol, Belgium
Sofie De Prins - VITO- Sustainable Health, Mol, Belgium
Tijs Louwies - VITO- Sustainable Health, Mol, Belgium
Vera Nelen - Environment and Health unit, Provincial Institute of Hygiene, Antwerp, Belgium
Guy Van Camp - University of Antwerp
Diether Lambrechts - KU Leuven
Greet Schoeters - University of Antwerp
Wim Vanden Berghe - University of Antwerp
Patrick De Boever - Hasselt University
Resource Type: Journal article
Publication Details: Clinical epigenetics, Vol.10(1), pp.50-50
DOI: 10.1186/s13148-018-0484-1
PMID: 29682088
PMCID: PMC5896137
NLM abbreviation: Clin Epigenetics
ISSN: 1868-7075
eISSN: 1868-7083
Grant note: DOI: 10.13039/501100001961, name: AXA Research Fund; name: CEFIC-LRI Innovative Science Award 2013; DOI: 10.13039/501100003130, name: Fonds Wetenschappelijk Onderzoek, award: 12L5216N; DOI: 10.13039/100000865, name: Bill and Melinda Gates Foundation, award: OPP119403
Language: English
Date published: 2018
Academic Unit: Psychiatry
Record Identifier: 9984281751602771

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