GLUT4 facilitates insulin stimulation and cAMP-mediated inhibition of glucose transport

J C Lawrence; R C Piper; L J Robinson; D E James

doi:10.1073/pnas.89.8.3493

Back

GLUT4 facilitates insulin stimulation and cAMP-mediated inhibition of glucose transport

Journal article

Open access

Peer reviewed

GLUT4 facilitates insulin stimulation and cAMP-mediated inhibition of glucose transport

J C Lawrence, R C Piper, L J Robinson and D E James

Proceedings of the National Academy of Sciences - PNAS, Vol.89(8), pp.3493-3497

04/15/1992

DOI: 10.1073/pnas.89.8.3493

PMCID: PMC48894

PMID: 1314390

Files and links (1)

url

https://doi.org/10.1073/pnas.89.8.3493View

Published (Version of record) Open Access

Abstract

The glucose transporter isoform GLUT4 is found only in cells that exhibit insulin-sensitive glucose transport. To investigate the function of this transporter, L6 myoblasts were stably transfected with GLUT4 cDNA. GLUT4 underwent insulin-dependent movement to the cell surface in myoblasts overexpressing the transporter. One cell line (243-6) expressed sufficient levels of the GLUT4 protein to study insulin-dependent glucose transport. Unlike wild-type L6 cells, 243-6 myoblasts exhibited two features that are characteristic of differentiated muscle fibers and adipocytes in vivo: a large insulin-stimulated component of glucose transport and inhibition of this stimulated component by cAMP. Relative to normal L6 cells, 243-6 cells responded to insulin or insulin-like growth factor 1 with a 5-fold larger increase in 2-deoxy[3H]glucose uptake. N6,O2'-Dibutyryladenosine 3',5'-cyclic monophosphate (Bt2cAMP) did not inhibit transport in normal L6 myoblasts, which express only GLUT1, but inhibited IGF-1/insulin-stimulated transport by 50% in 243-6 cells. The effect of cAMP was investigated further by using Chinese hamster ovary cells transiently expressing GLUT1 and GLUT4. Bt2cAMP inhibited glucose transport only in Chinese hamster ovary cells expressing GLUT4. These results indicate that cAMP-mediated inhibition of glucose transport is dependent on expression of the GLUT4 isozyme.

Details

Title: Subtitle: GLUT4 facilitates insulin stimulation and cAMP-mediated inhibition of glucose transport
Creators: J C Lawrence - Washington University in St. Louis
R C Piper - Washington University in St. Louis
L J Robinson - Washington University in St. Louis
D E James - Washington University in St. Louis
Resource Type: Journal article
Publication Details: Proceedings of the National Academy of Sciences - PNAS, Vol.89(8), pp.3493-3497
DOI: 10.1073/pnas.89.8.3493
PMID: 1314390
PMCID: PMC48894
NLM abbreviation: Proc Natl Acad Sci U S A
ISSN: 0027-8424
eISSN: 1091-6490
Publisher: National Academy of Sciences
Language: English
Date published: 04/15/1992
Academic Unit: Molecular Physiology and Biophysics; Medicine Administration; Internal Medicine
Record Identifier: 9984297500002771

Metrics

9 Record Views

31 Times Cited - Web of Science