Journal article
GM-CSF Increases AP-1 DNA Binding and Ref-1 Amounts in Human Alveolar Macrophages
American journal of respiratory cell and molecular biology, Vol.25(2), pp.254-259
08/2001
DOI: 10.1165/ajrcmb.25.2.4446
PMID: 11509337
Abstract
Alveolar macrophages have been implicated in the pathogenesis of a number of acute and chronic lung disorders. A characteristic feature of many of the chronic lung diseases is that the types of macrophages in the lung change, and in most instances, the cells resemble monocyte-like cells. We have previously shown that normal human alveolar macrophages have a decreased capacity to express protein kinase C (PKC)-induced DNA binding activity of the transcription factor activator protein (AP)-1 compared with monocytes. This decrease in AP-1 DNA binding appears to be due to a defect in redox regulation of AP-1 proteins via a decrease in the redox active protein Ref-1. The hypothesis for this study is that there are factors generated during the development of chronic lung disease that increase AP-1 DNA binding activity and Ref-1 production in human alveolar macrophages. We have focused specifically on granulocyte-macrophage colony-stimulating factor (GM-CSF) as a prototype mediator that can be released by alveolar macrophages and is related to the fibrotic process in the lung. We found that after a 24-h incubation with GM-CSF, AP-1 DNA binding was significantly increased in both unstimulated, interleukin (IL)-13, and phorbol myristate acetate (PMA)-stimulated alveolar macrophages and that there was a corresponding increase in Ref-1 protein by Western blot analysis in the PMA-stimulated group. This suggests that disease-related cytokines such as GM-CSF and IL-13 may modulate AP-1 DNA binding activity in alveolar macrophages.
Details
- Title: Subtitle
- GM-CSF Increases AP-1 DNA Binding and Ref-1 Amounts in Human Alveolar Macrophages
- Creators
- Dawn M FlahertyMartha M MonickA. Brent CarterMichael W PetersonGary W Hunninghake
- Resource Type
- Journal article
- Publication Details
- American journal of respiratory cell and molecular biology, Vol.25(2), pp.254-259
- DOI
- 10.1165/ajrcmb.25.2.4446
- PMID
- 11509337
- ISSN
- 1044-1549
- eISSN
- 1535-4989
- Language
- English
- Date published
- 08/2001
- Academic Unit
- Pulmonary, Critical Care, and Occupational Medicine; Radiation Oncology; Internal Medicine
- Record Identifier
- 9984094673802771
Metrics
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