Journal article
GPA1, a haploid-specific essential gene, encodes a yeast homolog of mammalian G protein which may be involved in mating factor signal transduction
Cell, Vol.50(7), pp.1011-1019
09/25/1987
DOI: 10.1016/0092-8674(87)90167-X
PMID: 3113739
Abstract
GPA1 protein of Saccharomyces cerevisiae is homologous to the alpha subunit of mammalian G protein. GPA1 transcript was found in haploid cells but was not detected in diploid cells. Disruption of GPA1 resulted in a haploid-specific lethal phenotype, indicating that GPA1 is a haploid-specific essential gene for cell growth. Upon regulation of expression of GPA1 by the galactose-inducible GAL1 promoter, the loss of GPA1 function was found to lead to cell-cycle arrest at the late G1 phase. Mutants that suppress the lethality of the gpa1::HIS3 mutation showed a sterile phenotype that was not cell-type-specific. These results suggest that GPA1 protein may control the signal for mating-factor-mediated cell-cycle arrest.
Details
- Title: Subtitle
- GPA1, a haploid-specific essential gene, encodes a yeast homolog of mammalian G protein which may be involved in mating factor signal transduction
- Creators
- Ikuko MiyajimaMasato NakafukuNaoki NakayamaCharles BrennerAtsushi MiyajimaKozo KaibuchiKen-ichi AraiYoshito KaziroKunihiro Matsumoto
- Resource Type
- Journal article
- Publication Details
- Cell, Vol.50(7), pp.1011-1019
- Publisher
- United States
- DOI
- 10.1016/0092-8674(87)90167-X
- PMID
- 3113739
- ISSN
- 0092-8674
- eISSN
- 1097-4172
- Language
- English
- Date published
- 09/25/1987
- Academic Unit
- Biochemistry and Molecular Biology; Internal Medicine
- Record Identifier
- 9983788432502771
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