Journal article
Galloyl moieties enhance the dentin biomodification potential of plant-derived catechins
Acta biomaterialia, Vol.10(7), pp.3288-3294
07/2014
DOI: 10.1016/j.actbio.2014.03.036
PMCID: PMC4041811
PMID: 24721612
Abstract
[Display omitted]
Proanthocyanidin-rich plant-derived agents have been shown to enhance dentin biomechanical properties and resistance to collagenase degradation. This study systematically investigated the interaction of chemically well-defined monomeric catechins with dentin extracellular matrix components by evaluating dentin mechanical properties as well as activities of matrix metalloproteinases (MMPs) and cysteine-cathepsins (CTs). Demineralized dentin beams (n=15) were incubated for 1h with 0.65% (+)-catechin (C), (−)-catechin gallate (CG), (−)-gallocatechin gallate (GCG), (−)-epicatechin (EC), (−)-epicatechin gallate (ECG), (−)-epigallocatechin (EGC) and (−)-epigallocatechin-3-gallate (EGCG). The modulus of elasticity (E) and the fold increase in E were determined by comparing specimens at baseline and after treatment. Biodegradation rates were assessed by differences in percentage of dry mass before and after incubation with bacterial collagenase. The inhibition of MMP-9 and CT-B by 0.65, 0.065 and 0.0065% of each catechin was determined using fluorimetric proteolytic assay kits. All monomeric catechins led to a significant increase in E. EGCG showed the highest fold increase in E, followed by ECG, CG and GCG. EGCG, ECG, GCG and CG significantly lowered biodegradation rates and inhibited both MMP-9 and CT-B at a concentration of 0.65%. Overall, the 3-O-galloylated monomeric catechins are clearly more potent than their non-galloylated analogues in improving dentin mechanical properties, stabilizing collagen against proteolytic degradation, and inhibiting the activity of MMPs and CTs. The results indicate that galloylation is a key pharmacophore in the monomeric and likely also in the oligomeric proanthocyanidins that exhibit high cross-linking potential for dentin extracellular matrix.
Details
- Title: Subtitle
- Galloyl moieties enhance the dentin biomodification potential of plant-derived catechins
- Creators
- Cristina M.P Vidal - Department of Restorative Dentistry, College of Dentistry, University of Illinois at Chicago, 801 South Paulina St, Chicago, IL 60612, USAThaiane R Aguiar - Department of Restorative Dentistry, College of Dentistry, University of Illinois at Chicago, 801 South Paulina St, Chicago, IL 60612, USARasika Phansalkar - Department of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, University of Illinois at Chicago, 833 South Wood St, Chicago, IL 60612, USAJames B McAlpine - Department of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, University of Illinois at Chicago, 833 South Wood St, Chicago, IL 60612, USAJosé G Napolitano - Department of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, University of Illinois at Chicago, 833 South Wood St, Chicago, IL 60612, USAShao-Nong Chen - Department of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, University of Illinois at Chicago, 833 South Wood St, Chicago, IL 60612, USALarissa S.N Araújo - Department of Restorative Dentistry, College of Dentistry, University of Illinois at Chicago, 801 South Paulina St, Chicago, IL 60612, USAGuido F Pauli - Department of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, University of Illinois at Chicago, 833 South Wood St, Chicago, IL 60612, USAAna Bedran-Russo - Department of Restorative Dentistry, College of Dentistry, University of Illinois at Chicago, 801 South Paulina St, Chicago, IL 60612, USA
- Resource Type
- Journal article
- Publication Details
- Acta biomaterialia, Vol.10(7), pp.3288-3294
- DOI
- 10.1016/j.actbio.2014.03.036
- PMID
- 24721612
- PMCID
- PMC4041811
- NLM abbreviation
- Acta Biomater
- ISSN
- 1742-7061
- eISSN
- 1878-7568
- Publisher
- Elsevier Ltd
- Language
- English
- Date published
- 07/2014
- Academic Unit
- Operative Dentistry
- Record Identifier
- 9984065702902771
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