Gamma protocadherins are required for survival of spinal interneurons

Xiaozhong Wang; Joshua A Weiner; Sabine Levi; Ann Marie Craig; Allan Bradley; Joshua R Sanes

doi:10.1016/S0896-6273(02)01090-5

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Gamma protocadherins are required for survival of spinal interneurons

Journal article

Open access

Peer reviewed

Gamma protocadherins are required for survival of spinal interneurons

Xiaozhong Wang, Joshua A Weiner, Sabine Levi, Ann Marie Craig, Allan Bradley and Joshua R Sanes

Neuron (Cambridge, Mass.), Vol.36(5), pp.843-854

12/05/2002

DOI: 10.1016/S0896-6273(02)01090-5

PMID: 12467588

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https://doi.org/10.1016/S0896-6273(02)01090-5View

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Abstract

The murine genome contains approximately 70 protocadherin (Pcdh) genes. Many are expressed in the nervous system, suggesting that Pcdhs may specify neuronal connectivity. Here, we analyze the 22 contiguous genes of the Pcdh-gamma cluster. Individual neurons express subsets of Pcdh-gamma genes. Pcdh-gamma proteins are present in most neurons and associated with, but not confined to, synapses. Early steps in neuronal migration, axon outgrowth, and synapse formation proceed in mutant mice lacking all 22 Pcdh-gamma genes. At late embryonic stages, however, dramatic neurodegeneration leads to neonatal death. In mutant spinal cord, many interneurons are lost, but sensory and motor neurons are relatively spared. In cultures from mutant spinal cord, neurons differentiate and form synapses but then die. Thus, Pcdh-gamma genes are dispensable for at least some aspects of connectivity but required for survival of specific neuronal types.

Immunohistochemistry

Interneurons - physiology

Cell Fractionation

Multigene Family

Retina - metabolism

Spinal Cord - metabolism

Cadherins - metabolism

Brain - physiology

Recombinant Fusion Proteins - metabolism

In Situ Hybridization

Protein Isoforms - metabolism

Synapses - metabolism

Embryo, Mammalian - anatomy & histology

Retina - cytology

Spinal Cord - pathology

Spinal Cord - cytology

Cadherins - genetics

Cell Differentiation - physiology

Cell Survival - physiology

Synapses - chemistry

Brain - cytology

Interneurons - ultrastructure

Cells, Cultured

Hippocampus - cytology

Mice, Knockout

Embryo, Mammalian - physiology

Hippocampus - metabolism

Animals

Recombinant Fusion Proteins - genetics

Mice

Protein Isoforms - genetics

Details

Title: Subtitle: Gamma protocadherins are required for survival of spinal interneurons
Creators: Xiaozhong Wang - Department of Molecular and Human Genetics, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA
Joshua A Weiner
Sabine Levi
Ann Marie Craig
Allan Bradley
Joshua R Sanes
Resource Type: Journal article
Publication Details: Neuron (Cambridge, Mass.), Vol.36(5), pp.843-854
DOI: 10.1016/S0896-6273(02)01090-5
PMID: 12467588
NLM abbreviation: Neuron
ISSN: 0896-6273
eISSN: 1097-4199
Publisher: United States
Grant note: DOI: 10.13039/100000002, name: National Institutes of Health
Language: English
Date published: 12/05/2002
Academic Unit: Liberal Arts and Science Admin; Psychiatry; Civil and Environmental Engineering; Iowa Neuroscience Institute; Biology
Record Identifier: 9983997988902771

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