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Gas chromatographic separation of methoxylated polychlorinated biphenyl atropisomers
Journal article   Peer reviewed

Gas chromatographic separation of methoxylated polychlorinated biphenyl atropisomers

Izabela Kania-Korwel, Sandhya M. Vyas, Yang Song and Hans-Joachim Lehmler
JOURNAL OF CHROMATOGRAPHY A, Vol.1207(1-2), pp.146-154
10/17/2008
DOI: 10.1016/j.chroma.2008.08.044
PMCID: PMC2579784
PMID: 18760792
url
https://www.ncbi.nlm.nih.gov/pmc/articles/2579784View
Open Access

Abstract

Several polychlorinated biphenyls (PCBs) and their hydroxylated metabolites display axial chirality. Here we describe an enantioselective, gas chromatographic separation of methylated derivatives of hydroxylated (OH-)PCB atropisomers (MeO-PCB) using a chemically bonded beta-cyclodextrin column (Chirasil-Dex). The atropisomers of several MeO-PCBs could be separated on this column with resolutions ranging from 0.42 to 0.87 under isothermal or temperature-programmed conditions. In addition, the enantiomeric fraction of OH-PCB 136 metabolites was determined in male and female rats treated with racemic PCB 136. The methylated derivatives of two OH-PCB 136 metabolites showed an enantiomeric enrichment in liver tissue, whereas PCB 136 itself was near racemic. (C) 2008 Elsevier B.V. All rights reserved.
Biochemical Research Methods Biochemistry & Molecular Biology Chemistry Chemistry, Analytical Life Sciences & Biomedicine Physical Sciences Science & Technology

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