Journal article
Gas-phase fragmentations of anionic complexes of serine- and threonine-containing peptides
International journal of mass spectrometry and ion processes, Vol.127(C), pp.17-26
08/19/1993
DOI: 10.1016/0168-1176(93)87075-4
Abstract
The (M + Ca − 3H)
− and (M − H)
− anions of 11 tetra- and larger peptides that contain either Ser or Thr are studied by using fast atom bombardment (FAB) and collision-induced decomposition (CID). The Ser- and Thr-containing negative ions decompose to lose either 30 u (Ser) or 44 u (Thr) in the ion source, metastably, and upon CID. The CID of the FAB-formed (M + Ca − 3H − X)
− or (M − H − X)
− fragment ions, in which X = 30 or 44 u, is the same as the CID of (M + Ca − 3H)
− or (M − H)
− anions of peptides in which the Ser and Thr residues are replaced with Gly. Two possible mechanisms for the decompositions are discussed, and theoretical molecular mechanical calculations provide further evidence regarding the mechanisms.
Details
- Title: Subtitle
- Gas-phase fragmentations of anionic complexes of serine- and threonine-containing peptides
- Creators
- Alex Reiter - Emory UniversityLynn M. Teesch - Emory UniversityHong ZhaoJeanette Adams - Emory University
- Resource Type
- Journal article
- Publication Details
- International journal of mass spectrometry and ion processes, Vol.127(C), pp.17-26
- Publisher
- Elsevier B.V
- DOI
- 10.1016/0168-1176(93)87075-4
- ISSN
- 0168-1176
- eISSN
- 1873-2801
- Language
- English
- Date published
- 08/19/1993
- Academic Unit
- Core Research Facilities; Medicine Administration
- Record Identifier
- 9984622756502771
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