Journal article
Gas-sensing internal enzyme fiber optic biosensor for hydrogen peroxide
Talanta (Oxford), Vol.41(5), pp.783-787
1994
DOI: 10.1016/0039-9140(93)E0065-L
PMID: 18965999
Abstract
Feasibility is demonstrated for a novel gas-sensing, internal enzyme biosensing scheme for the selective measurement of hydrogen peroxide. Two horseradish peroxidase catalysed reactions are evaluated for the detection of hydrogen peroxide as it crosses a microporous Teflon membrane at 37°C. The rate at which hydrogen peroxide crosses the membrane is determined by either a fluorescence or chemiluminescence measurement and this rate is related to the concentration of hydrogen peroxide in the sample solution. Detection limits of 0.7 m
M and 10 μ
M are estimated for the fluorescence and chemiluminescence methods, respectively. Selectivity is demonstrated for hydrogen peroxide over ascorbic acid, uric acid and tyrosine.
Details
- Title: Subtitle
- Gas-sensing internal enzyme fiber optic biosensor for hydrogen peroxide
- Creators
- Mark A ArnoldXiangji ZhouRebecca S Petsch
- Resource Type
- Journal article
- Publication Details
- Talanta (Oxford), Vol.41(5), pp.783-787
- DOI
- 10.1016/0039-9140(93)E0065-L
- PMID
- 18965999
- NLM abbreviation
- Talanta
- ISSN
- 0039-9140
- eISSN
- 1873-3573
- Publisher
- Elsevier B.V
- Language
- English
- Date published
- 1994
- Academic Unit
- Center for Biocatalysis and Bioprocessing; Fraternal Order of Eagles Diabetes Research Center; Chemistry
- Record Identifier
- 9984217457402771
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