Journal article
Gastrointestinal Transit during Endotoxemia: The Role of Nitric Oxide
The Journal of surgical research, Vol.60(2), pp.307-311
02/01/1996
DOI: 10.1006/jsre.1996.0048
PMID: 8598659
Abstract
We hypothesized that the disrupted gastrointestinal transit that occurs during endotoxemia is mediated by nitric oxide (NO) and that the inhibition of NO synthesis will normalize intestinal transit and gastric emptying. To determine the effects of endotoxin and steroids on the activity of gastrointestinal smooth muscle NO synthase, rats underwent placement of an intravenous (iv) line and then were givenEscherichia colilipopolysaccharide (LPS), 10 mg/kg/iv; LPS, 10 mg/kg/iv + dexamethasone, 3 mg/kg/iv; or saline. The activity of nitric oxide synthase in the stomach, small intestine, and colon were determined by measuring the conversion ofL-[3H]arginine toL-[3H]citrulline. To determine intestinal transit and gastric emptying, gavage feedings of nonabsorbable liquid markers were given and rats divided into eight groups: 0.9% NaCl, 1 ml/hr × 5 hr (control); LPS, 10 mg/kg/iv; LPS +Nω-nitro-L-arginine methyl ester (L-NAME), 10 mg/kg/hr × 5 hr; LPS +Nω-nitro-D-arginine methyl ester (D-NAME), 10 mg/kg/hr × 5 hr; LPS +L-arginine, 100 mg/kg/hr × 5 hr; LPS +L-NAME +L-arginine; LPS +Nω-nitro-L-arginine (L-NNA), 10 mg/kg/hr; or LPS +L-NNA +L-arginine. LPS increased the enzymatic activity of both the constitutive and the inducible forms of NO synthase in the small intestine and fundus of the stomach. The acceleration of intestinal transit produced by endotoxemia was reversed with bothL-NAME andL-NNA but not withD-NAME. Endotoxemia slowed gastric emptying but this effect was not reversed with eitherL-NAME orL-NNA. We conclude that NO plays a major role in mediating the rapid intestinal transit during endotoxemia.
Details
- Title: Subtitle
- Gastrointestinal Transit during Endotoxemia: The Role of Nitric Oxide
- Creators
- Douglas J Wirthlin - Department of Surgery, University of Iowa College of Medicine, Iowa City, Iowa, 52242Joseph J Cullen - Department of Surgery, University of Iowa College of Medicine, Iowa City, Iowa, 52242Stephen T Spates - Surgical Service, VAMC, Iowa City, Iowa, 52242Jeffrey L Conklin - Department of Internal Medicine, University of Iowa College of Medicine, Iowa City, Iowa, 52242Joseph Murray - Department of Internal Medicine, University of Iowa College of Medicine, Iowa City, Iowa, 52242David K Caropreso - Surgical Service, VAMC, Iowa City, Iowa, 52242Kimberly S Ephgrave - Department of Surgery, University of Iowa College of Medicine, Iowa City, Iowa, 52242
- Resource Type
- Journal article
- Publication Details
- The Journal of surgical research, Vol.60(2), pp.307-311
- Publisher
- Elsevier Inc
- DOI
- 10.1006/jsre.1996.0048
- PMID
- 8598659
- ISSN
- 0022-4804
- eISSN
- 1095-8673
- Language
- English
- Date published
- 02/01/1996
- Academic Unit
- Surgery; Radiation Oncology
- Record Identifier
- 9984051525602771
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