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Gene Conversion and Crossing Over Along the 405-kb Left Arm of Saccharomyces cerevisiae Chromosome VII
Journal article   Open access   Peer reviewed

Gene Conversion and Crossing Over Along the 405-kb Left Arm of Saccharomyces cerevisiae Chromosome VII

Anna Malkova, Johanna Swanson, Miriam German, John H McCusker, Elizabeth A Housworth, Franklin W Stahl and James E Haber
Genetics (Austin), Vol.168(1), pp.49-63
09/2004
DOI: 10.1534/genetics.104.027961
PMCID: PMC1448106
PMID: 15454526
url
https://doi.org/10.1534/genetics.104.027961View
Published (Version of record) Open Access

Abstract

Gene conversions and crossing over were analyzed along 10 intervals in a 405-kb region comprising nearly all of the left arm of chromosome VII in Saccharomyces cerevisiae . Crossover interference was detected in all intervals as measured by a reduced number of nonparental ditypes. We have evaluated interference between crossovers in adjacent intervals by methods that retain the information contained in tetrads as opposed to single segregants. Interference was seen between intervals when the distance in the region adjacent to a crossover was <∼35 cM (90 kb). At the met13 locus, which exhibits ∼9% gene conversions, those gene conversions accompanied by crossing over exerted interference in exchanges in an adjacent interval, whereas met13 gene conversions without an accompanying exchange did not show interference. The pattern of exchanges along this chromosome arm can be represented by a counting model in which there are three nonexchange events between adjacent exchanges; however, maximum-likelihood analysis suggests that ∼8–12% of the crossovers on chromosome VII arise by a separate, noninterfering mechanism.
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