Journal article
Gene Delivery to the Airway
Current Protocols in Human Genetics, Vol.23(1), pp.13.9.1-13.9.34
11/1999
DOI: 10.1002/0471142905.hg1309s23
PMID: 18428257
Abstract
This unit describes generation of and gene transfer to several commonly used airway models. Isolation and transduction of primary airway epithelial cells are first described. Next, the preparation of polarized airway epithelial monolayers is outlined. Transduction of these polarized cells is also described. Methods are presented for generation of human bronchial xenografts as well as both ex vivo and in vivo gene transfer to these xenografts. Finally, a method for in vivo gene delivery to the lungs of rodents is included. Methods for evaluating transgene expression are given in the support protocols.
Details
- Title: Subtitle
- Gene Delivery to the Airway
- Creators
- Dongsheng Duan - University of IowaYulong Zhang - University of IowaJohn F Engelhardt - University of Iowa
- Resource Type
- Journal article
- Publication Details
- Current Protocols in Human Genetics, Vol.23(1), pp.13.9.1-13.9.34
- DOI
- 10.1002/0471142905.hg1309s23
- PMID
- 18428257
- ISSN
- 1934-8266
- eISSN
- 1934-8258
- Number of pages
- 34
- Language
- English
- Date published
- 11/1999
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Anatomy and Cell Biology; Radiation Oncology; Internal Medicine
- Record Identifier
- 9984025461202771
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