Journal article
Gene Expression Profiles Reveal that Chondrogenic Progenitor Cells and Synovial Cells are Closely Related
Journal of orthopaedic research, Vol.32(8), pp.981-988
08/2014
DOI: 10.1002/jor.22641
PMCID: PMC6415308
PMID: 24797716
Abstract
We showed previously that chondrogenic progenitor cells (CPCs) from the superficial zone of articular cartilage respond vigorously to cartilage wounding by responding chemotactically to cell debris, but the physiologic functions of CPCs remain unclear. To help bridge this knowledge gap we undertook a comparative analysis of gene expression in bovine CPCs, chondrocytes, synovial fibroblasts (synoviocytes), and cells isolated from synovial fluid (SFCs). Analysis of microarrays parsed the four cell types into two distinct groups, one composed only of chondrocytes and the other of CPCs, synoviocytes and SFCs. The groups differed with respect to metalloendopeptidase, collagen, and cytokine gene expression. Quantitative PCR showed that, relative to chondrocytes, all other cells under-expressed cartilage matrix genes. CPCs significantly over-expressed genes encoding the chemokines interleukin 8 (IL8), and C-C motif ligand 2, while synoviocytes over-expressed the chemokine C-X-C motif Ligand 12. Sulfated glycosaminoglycan deposition in pellet cultures by CPCs was intermediate between chondrocytes and synoviocytes/SFCs. These results indicate that the CPC phenotype more closely resembles synoviocytes and SFCs than chondrocytes. CPCs show a tendency to over-express chemokines that promote immune cell chemotaxis, suggesting they mediate inflammation in response to cartilage wounding.
Details
- Title: Subtitle
- Gene Expression Profiles Reveal that Chondrogenic Progenitor Cells and Synovial Cells are Closely Related
- Creators
- Cheng Zhou - Department of Orthopaedics and Rehabilitation, University of Iowa, Iowa City, IA 52242Hongjun Zheng - Department of Orthopaedics and Rehabilitation, University of Iowa, Iowa City, IA 52242Dongrim Seol - Department of Orthopaedics and Rehabilitation, University of Iowa, Iowa City, IA 52242Yin Yu - Department of Orthopaedics and Rehabilitation, University of Iowa, Iowa City, IA 52242James A Martin - Department of Orthopaedics and Rehabilitation, University of Iowa, Iowa City, IA 52242
- Resource Type
- Journal article
- Publication Details
- Journal of orthopaedic research, Vol.32(8), pp.981-988
- DOI
- 10.1002/jor.22641
- PMID
- 24797716
- PMCID
- PMC6415308
- ISSN
- 0736-0266
- eISSN
- 1554-527X
- Grant note
- DOI: 10.13039/100000002, name: National Institutes of Health, award: CORT NIH P50 AR055533; DOI: 10.13039/100000005, name: Department of Defense, award: W81XWH-10-1-0702a; name: American Arthritis Society
- Language
- English
- Date published
- 08/2014
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Orthodontics; Pharmaceutical Sciences and Experimental Therapeutics; Orthopedics and Rehabilitation
- Record Identifier
- 9984040226702771
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