Gene Expression Profiling of Facilitated L-LTP in VP16-CREB Mice Reveals that BDNF Is Critical for the Maintenance of LTP and Its Synaptic Capture

Angel Barco; Susan Patterson; Juan M. Alarcon; Petra Gromova; Manuel Mata-Roig; Alexei Morozov; Eric R Kandel

doi:10.1016/j.neuron.2005.09.005

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Gene Expression Profiling of Facilitated L-LTP in VP16-CREB Mice Reveals that BDNF Is Critical for the Maintenance of LTP and Its Synaptic Capture

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Gene Expression Profiling of Facilitated L-LTP in VP16-CREB Mice Reveals that BDNF Is Critical for the Maintenance of LTP and Its Synaptic Capture

Angel Barco, Susan Patterson, Juan M. Alarcon, Petra Gromova, Manuel Mata-Roig, Alexei Morozov and Eric R Kandel

Neuron (Cambridge, Mass.), Vol.48(1), pp.123-137

10/06/2005

DOI: 10.1016/j.neuron.2005.09.005

PMID: 16202713

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Abstract

Expression of VP16-CREB, a constitutively active form of CREB, in hippocampal neurons of the CA1 region lowers the threshold for eliciting the late, persistent phase of long-term potentiation (L-LTP) in the Schaffer collateral pathway. This VP16-CREB-mediated L-LTP differs from the conventional late phase of LTP in not being dependent on new transcription. This finding suggests that in the transgenic mice the mRNA transcript(s) encoding the protein(s) necessary for this form of L-LTP might already be present in CA1 neurons in the basal condition. We used high-density oligonucleotide arrays to identify the mRNAs differentially expressed in the hippocampus of transgenic and wild-type mice. We then explored the contribution of the most prominent candidate genes revealed by our screening, namely prodynorphin, BDNF, and MHC class I molecules, to the facilitated LTP of VP16-CREB mice. We found that the overexpression of brain-derived neurotrophic factor accounts for an important component of this phenotype.

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Title: Subtitle: Gene Expression Profiling of Facilitated L-LTP in VP16-CREB Mice Reveals that BDNF Is Critical for the Maintenance of LTP and Its Synaptic Capture
Creators: Angel Barco - Center for Neurobiology and Behavior, Columbia University, 1051 Riverside Drive, New York, New York 10032
Susan Patterson
Juan M. Alarcon - Center for Neurobiology and Behavior, Columbia University, 1051 Riverside Drive, New York, New York 10032
Petra Gromova - Instituto de Neurociencias de Alicante, Universidad Miguel Hernandez, San Juan de Alicante 03550, Spain
Manuel Mata-Roig - Unidad Central de Investigación, Facultad de Medicina, Universidad de Valencia, Valencia 46010, Spain
Alexei Morozov - Center for Neurobiology and Behavior, Columbia University, 1051 Riverside Drive, New York, New York 10032
Eric R Kandel - Center for Neurobiology and Behavior, Columbia University, 1051 Riverside Drive, New York, New York 10032
Resource Type: Journal article
Publication Details: Neuron (Cambridge, Mass.), Vol.48(1), pp.123-137
DOI: 10.1016/j.neuron.2005.09.005
PMID: 16202713
NLM abbreviation: Neuron
ISSN: 0896-6273
eISSN: 1097-4199
Publisher: Elsevier Inc
Language: English
Date published: 10/06/2005
Academic Unit: Iowa Neuroscience Institute
Record Identifier: 9984454746202771

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