Journal article
Gene delivery to the airway
Current protocols in human genetics, Vol.Chapter 13(1), pp.Unit 13.9-13.9.41
07/2013
DOI: 10.1002/0471142905.hg1309s78
PMCID: PMC3777227
PMID: 23853081
Abstract
This unit describes generation of and gene transfer to several commonly used airway models. Isolation and transduction of primary airway epithelial cells are first described. Next, the preparation of polarized airway epithelial monolayers is outlined. Transduction of these polarized cells is also described. Methods are presented for generation of tracheal xenografts, as well as both ex vivo and in vivo gene transfer to these xenografts. Finally, a method for in vivo gene delivery to the lungs of rodents is included. Methods for evaluating transgene expression are given in the support protocols.
Details
- Title: Subtitle
- Gene delivery to the airway
- Creators
- Nicholas W Keiser - University of Iowa, Iowa City, Iowa, USAJohn F Engelhardt
- Resource Type
- Journal article
- Publication Details
- Current protocols in human genetics, Vol.Chapter 13(1), pp.Unit 13.9-13.9.41
- DOI
- 10.1002/0471142905.hg1309s78
- PMID
- 23853081
- PMCID
- PMC3777227
- NLM abbreviation
- Curr Protoc Hum Genet
- ISSN
- 1934-8266
- eISSN
- 1934-8258
- Publisher
- United States
- Grant note
- R01 HL108902 / NHLBI NIH HHS P30 ES005605 / NIEHS NIH HHS DK047967 / NIDDK NIH HHS HL108902 / NHLBI NIH HHS R37 DK047967 / NIDDK NIH HHS R01 DK047967 / NIDDK NIH HHS P30 DK054759 / NIDDK NIH HHS DK54759 / NIDDK NIH HHS
- Language
- English
- Date published
- 07/2013
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Anatomy and Cell Biology; Radiation Oncology; Internal Medicine
- Record Identifier
- 9984025354302771
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