Journal article
Gene expression profiling analysis of the inner ear
Hearing research, Vol.225(1), pp.1-10
2007
DOI: 10.1016/j.heares.2007.01.015
PMID: 17300888
Abstract
Recent developments in molecular genetics, including progress in the human genome project, have allowed identification of genes at an unprecedented rate. To date gene expression profiling studies have focused on identifying transcripts that are specifically or preferentially enriched within the inner ear on the assumption that they are more likely to be important for auditory and vestibular function. It is now apparent that some genes preferentially expressed in the cochleo-vestibular system are not crucial for hearing or balance or their functions are compensated for by other genes. In addition, transcripts expressed at low abundance in the inner ear are generally under-represented in gene profiling studies. In this review, we highlight the limitations of current gene expression profiling strategies as a discovery tool for genes involved in cochleo-vestibular development and function. We argue that expression profiling based on hierarchical clustering of transcripts by gene ontology, combined with tissue enrichment data, is more effective for inner ear gene discovery. This approach also provides a framework to assist and direct the functional characterization of gene products.
Details
- Title: Subtitle
- Gene expression profiling analysis of the inner ear
- Creators
- Michael S Hildebrand - Department of Otolaryngology – Head and Neck Surgery, University of Iowa, Iowa City, IA 52242, USAMichelle G de Silva - Baker Medical Research Institute, The Alfred Hospital, Melbourne, AustraliaTuomas Klockars - Department of Othorhinolaryngology, Helsinki University Central Hospital, Helsinki, FinlandColleen A Campbell - Department of Otolaryngology – Head and Neck Surgery, University of Iowa, Iowa City, IA 52242, USARichard J.H Smith - Department of Otolaryngology – Head and Neck Surgery, University of Iowa, Iowa City, IA 52242, USAHans-Henrik M Dahl - Murdoch Childrens Research Institute, Royal Children’s Hospital, Melbourne, Australia
- Resource Type
- Journal article
- Publication Details
- Hearing research, Vol.225(1), pp.1-10
- DOI
- 10.1016/j.heares.2007.01.015
- PMID
- 17300888
- NLM abbreviation
- Hear Res
- ISSN
- 0378-5955
- eISSN
- 1878-5891
- Publisher
- Elsevier B.V
- Language
- English
- Date published
- 2007
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Molecular Physiology and Biophysics; Anatomy and Cell Biology; Stead Family Department of Pediatrics; Iowa Neuroscience Institute; Cardiovascular Medicine; Otolaryngology; Internal Medicine
- Record Identifier
- 9984007292902771
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