Journal article
Gene transfer to the nonhuman primate retina with recombinant feline immunodeficiency virus vectors
Human gene therapy, Vol.13(6), pp.689-696
04/10/2002
DOI: 10.1089/104303402317322258
PMID: 11936968
Abstract
We hypothesize that recombinant feline immunodeficiency viral (rFIV) vectors may be useful for gene transfer to the nonhuman primate retina. We performed vitrectomies and subretinal injections in the right eyes of 11 cynomolgus monkeys. Vesicular stomatitis virus glycoprotein-pseudotyped rFIV that expressed the Escherichia coli beta-galactosidase gene was injected into eight eyes. Sham vehicle or lactose buffer injections were also performed in two of these eight study eyes. rFIV pseudotyped with an amphotropic envelope was used in two eyes, and in one animal injections of lactose buffer only were given. After surgery the animals were clinically evaluated by retinal photography and electroretinography. beta-Galactosidase expression was evaluated, at a final end point, in histological sections. We found photoreceptor and Müller cells to have the greatest transgene expression. Focal inflammatory responses localized to the injection site were seen histologically in all eyes. No difference in transduction efficiency was seen between injections near the macula and more peripheral injections. Visual function as assessed by electroretinography was not significantly affected by vector or vehicle injections. We conclude that rFIV vectors administered beneath the retina can transduce a variety of retinal cells in the nonhuman primate retina. rFIV vectors have therapeutic potential and could be exploited to develop gene therapy for the human eye.
Details
- Title: Subtitle
- Gene transfer to the nonhuman primate retina with recombinant feline immunodeficiency virus vectors
- Creators
- Andrew J Lotery - Department of Ophthalmology and Visual Sciences, University of Iowa Hospitals and Clinics, 200 Hawkins Drive, Iowa City, IA 52242, USA. andrew-lotery@uiowa.eduTodd A DerksenStephen R RussellRobert F MullinsSybille SauterLouisa M AffatigatoEdwin M StoneBeverly L Davidson
- Resource Type
- Journal article
- Publication Details
- Human gene therapy, Vol.13(6), pp.689-696
- DOI
- 10.1089/104303402317322258
- PMID
- 11936968
- NLM abbreviation
- Hum Gene Ther
- ISSN
- 1043-0342
- eISSN
- 1557-7422
- Publisher
- United States
- Grant note
- P30 DK 54759 / NIDDK NIH HHS NS 34568 / NINDS NIH HHS
- Language
- English
- Date published
- 04/10/2002
- Academic Unit
- The University of Iowa Institute for Vision Research; Iowa Neuroscience Institute; Ophthalmology and Visual Sciences
- Record Identifier
- 9983979917602771
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