Journal article
Generalized Two-Dimensional Heterocorrelation Analysis of Spectrally Resolved and Temporally Resolved Fluorescence of the 8-Anilino-1-naphthalenesulfonate−Apomyoglobin Complex with pH Perturbation
The journal of physical chemistry. B, Vol.110(16), pp.8506-8512
04/01/2006
DOI: 10.1021/jp0555293
PMID: 16623538
Abstract
We demonstrate two-dimensional heterocorrelation analysis between spectrally resolved and temporally resolved fluorescence to investigate the decay dynamics of the 8-anilino-1-naphthalenesulfonate - (ANS - ) apomyoglobin complex. The dynamic changes of the lifetime components are disclosed across the emission spectrum with an external pH-perturbation. Two different fluorescence lifetime schemes of the ANS-apomyoglobin complex are revealed. From pH 8.5 to 4.5, the transition of protein conformation from the native state to the folding intermediate, a short lifetime component is found to correlate with a short-wavelength emission whose population diminishes with decreasing pH. The lifetime components reflect the excited-state populations of the nascent and the charge-transfer species. From pH 4.2 to 1.0, the transition from the folding intermediate to the acid-unfolded state, the short lifetime is responsible for a long-wavelength emission and the fraction of this component increases when the solution becomes more acidic. In this pH range, the decay components reflect the ground-state populations of microenvironments. The relative decay dynamics across the emission spectrum are revealed without collecting decays at each wavelength. More importantly, these conclusions are reached without the necessity of statistical fitting of the decay data with an a priori decay model. © 2006 American Chemical Society.
Details
- Title: Subtitle
- Generalized Two-Dimensional Heterocorrelation Analysis of Spectrally Resolved and Temporally Resolved Fluorescence of the 8-Anilino-1-naphthalenesulfonate−Apomyoglobin Complex with pH Perturbation
- Creators
- Gufeng Wang - Department of Chemistry, the Optical Science and Technology Center, and the Center for Biocatalysis and Bioprocessing, University of Iowa, Iowa City, Iowa 52242Yi Gao - Department of Chemistry, the Optical Science and Technology Center, and the Center for Biocatalysis and Bioprocessing, University of Iowa, Iowa City, Iowa 52242M. Lei Geng - Department of Chemistry, the Optical Science and Technology Center, and the Center for Biocatalysis and Bioprocessing, University of Iowa, Iowa City, Iowa 52242
- Resource Type
- Journal article
- Publication Details
- The journal of physical chemistry. B, Vol.110(16), pp.8506-8512
- DOI
- 10.1021/jp0555293
- PMID
- 16623538
- NLM abbreviation
- J Phys Chem B
- ISSN
- 1520-6106
- eISSN
- 1520-5207
- Publisher
- American Chemical Society
- Language
- English
- Date published
- 04/01/2006
- Academic Unit
- Chemistry
- Record Identifier
- 9984217455302771
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