Journal article
Generating iPSC-Derived Choroidal Endothelial Cells to Study Age-Related Macular Degeneration
Investigative ophthalmology & visual science, Vol.56(13), pp.8258-8267
12/2015
DOI: 10.1167/iovs.15-17073
PMCID: PMC5110238
PMID: 26720480
Abstract
Age-related macular degeneration (AMD), the most common cause of incurable blindness in the western world, is characterized by the dysfunction and eventual death of choroidal endothelial (CECs), RPE, and photoreceptor cells. Stem cell-based treatment strategies designed to replace photoreceptor and RPE cells currently are a major scientific focus. However, the success of these approaches likely also will require replacement of the underlying, supportive choroidal vasculature. The purpose of this study was to generate stem cell-derived CECs to develop efficient differentiation and transplantation protocols. Dermal fibroblasts from the Tie2-GFP mouse were isolated and reprogrammed into two independent induced pluripotent stem cell (iPSC) lines via viral transduction of the transcription factors Oct4, Sox2, Klf4, and c-Myc. Tie2-GFP iPSCs were differentiated into CECs using a coculture method with either the RF6A CEC line or primary mouse CECs. Induced pluripotent stem cell-derived CECs were characterized via RT-PCR and immunocytochemistry for EC- and CEC-specific markers. Induced pluripotent stem cells generated from mice expressing green fluorescent protein (GFP) under control of the endothelial Tie2 promoter display classic pluripotency markers and stem cell morphology. Induced pluripotent stem cell-derived CECs express carbonic anhydrase IV, eNOS, FOXA2, PLVAP, CD31, CD34, ICAM-1, Tie2, TTR, VE-cadherin, and vWF. Induced pluripotent stem cell-derived CECs will be a valuable tool for modeling of choriocapillaris-specific insults in AMD and for use in future choroidal endothelial cell replacement approaches.
Details
- Title: Subtitle
- Generating iPSC-Derived Choroidal Endothelial Cells to Study Age-Related Macular Degeneration
- Creators
- Allison E Songstad - University of IowaLuke A Wiley - University of IowaKhahn Duong - Department of Internal Medicine, University of Iowa, Iowa City, Iowa, United StatesEmily Kaalberg - University of IowaMiles J Flamme-Wiese - University of IowaCathryn M Cranston - University of IowaMegan J Riker - University of IowaDana Levasseur - Department of Internal Medicine, University of Iowa, Iowa City, Iowa, United StatesEdwin M Stone - University of IowaRobert F Mullins - University of IowaBudd A Tucker - University of Iowa
- Resource Type
- Journal article
- Publication Details
- Investigative ophthalmology & visual science, Vol.56(13), pp.8258-8267
- DOI
- 10.1167/iovs.15-17073
- PMID
- 26720480
- PMCID
- PMC5110238
- NLM abbreviation
- Invest Ophthalmol Vis Sci
- ISSN
- 0146-0404
- eISSN
- 1552-5783
- Publisher
- United States
- Grant note
- R01 EY024605 / NEI NIH HHS EY-024605 / NEI NIH HHS DP2 OD007483 / NIH HHS 1-DP2-OD007483-01 / NIH HHS
- Language
- English
- Date published
- 12/2015
- Academic Unit
- Iowa Neuroscience Institute; Ophthalmology and Visual Sciences
- Record Identifier
- 9983980070202771
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