Journal article
Generation and Characterization of Monoclonal Antibodies to NIAM: A Nuclear Interactor of ARF and Mdm2
Hybridoma (2005), Vol.27(3), pp.159-166
06/2008
DOI: 10.1089/hyb.2007.0533
PMCID: PMC3186703
PMID: 18582208
Abstract
Nuclear interactor of ARF and Mdm2 (NIAM) is a newly discovered growth inhibitor that helps maintain chromosomal stability. It is functionally linked to the ARF-Mdm2-p53 tumor suppressor pathway and is predicted to be a tumor suppressor, but the lack of antibodies capable of detecting the endogenous human protein has delayed efforts to define its role in human tumorigenesis. This study reports the development, screening, and characterization of several monoclonal antibodies (MAbs) that specifically recognize endogenous human NIAM protein by Western blotting, immunoprecipitation, immunofluorescence, and immunohistochemistry. These MAbs are predicted to be important tools for evaluating the expression and physiological function of NIAM in normal versus neoplastic human cells and tissues.
Details
- Title: Subtitle
- Generation and Characterization of Monoclonal Antibodies to NIAM: A Nuclear Interactor of ARF and Mdm2
- Creators
- Jussara Hagen - Department of PharmacologyVan Tompkins - Department of PharmacologyAmel Dudakovic - Department of PharmacologyJamie A Weydert - Department of PathologyDawn E Quelle - Department of Pharmacology
- Resource Type
- Journal article
- Publication Details
- Hybridoma (2005), Vol.27(3), pp.159-166
- DOI
- 10.1089/hyb.2007.0533
- PMID
- 18582208
- PMCID
- PMC3186703
- NLM abbreviation
- Hybridoma (Larchmt)
- ISSN
- 1554-0014
- eISSN
- 1557-8348
- Publisher
- Mary Ann Liebert, Inc
- Language
- English
- Date published
- 06/2008
- Academic Unit
- Molecular Physiology and Biophysics; Pathology; Neuroscience and Pharmacology
- Record Identifier
- 9984040556502771
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