Journal article
Generation and Characterization of a Low-Degree Drug-Resistant Human Tumor Cell Line
Oncology, Vol.47(6), pp.488-494
1990
DOI: 10.1159/000226878
PMID: 2243668
Abstract
A2780 human ovarian cancer cells, obtained from an untreated patient, have been exposed to a relatively low, clinically maintainable dose (10 nmol/l) of the anthracycline doxorubicin (DX) to derive a low-degree (5-fold) drug-resistant subline (A2780-DX1). Compared to parental cells, these DX-resistant cells have increased size (+60% of cell volume) and contain a greater number of cytoplasmic vacuoles as determined by electron microscopy. When exposed to several other antiproliferative drugs, A2780-DX1 cells were highly cross-resistant (>10-fold) to epirubicin, mafosfamide and cisplatin and slightly cross-resistant (2- to 3-fold) to navelbine and bleomycin, while they retained the original sensitivity to vinblastine, Ara-C and fluorouracil. Gel electrophoresis of cytoplasmic membrane proteins showed differences between the pattern of parental A2780 sensitive and A2780-DX1 cells as far as low-molecular-weight proteins (<45 kD) are concerned, while no clear overexpression of P-glycoprotein (P-170) could be detected. Membrane modifications yielding a decrease of both DX uptake and retention, increased content of intracellular glutathione (+32%) and reduced DNA double-strand breaks seem to be involved in the resulting multidrug-resistant phenotype of A2780-DX1 cells.
Details
- Title: Subtitle
- Generation and Characterization of a Low-Degree Drug-Resistant Human Tumor Cell Line
- Creators
- Alessandra Mazzoni - Istituto Nazionale per la Ricerca sul Cancro, Genova, Italia.Fabio TravePatrizia RussoAngelo NicolinYoucef M Rustum
- Resource Type
- Journal article
- Publication Details
- Oncology, Vol.47(6), pp.488-494
- DOI
- 10.1159/000226878
- PMID
- 2243668
- ISSN
- 0030-2414
- eISSN
- 1423-0232
- Number of pages
- 7
- Language
- English
- Date published
- 1990
- Academic Unit
- Hematology, Oncology, and Blood & Marrow Transplantation; Internal Medicine
- Record Identifier
- 9984359900902771
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