Journal article
Generation and characterization of new alleles of quiver (qvr) that encodes an extracellular modulator of the Shaker potassium channel
Journal of neurogenetics, Vol.31(4), pp.325-336
10/02/2017
DOI: 10.1080/01677063.2017.1393076
PMCID: PMC5918287
PMID: 29117754
Abstract
Our earlier genetic screen uncovered a paraquat-sensitive leg-shaking mutant quiver\n1\n(qvr\n1\n), whose gene product interacts with the Shaker (Sh) K\n+\nchannel. We also mapped the qvr locus to EY04063 and noticed altered day-night activity patterns in these mutants. Such circadian behavioral defects were independently reported by another group, who employed the qvr\n1\nallele we supplied them, and attributed the extreme restless phenotype of EY04063 to the qvr gene. However, their report adopted a new noncanonical gene name sleepless (sss) for qvr. In addition to qvr\n1\nand qvr\nEY\n, our continuous effort since the early 2000s generated a number of novel recessive qvr alleles, including ethyl methanesulfonate (EMS)-induced mutations qvr\n2\nand qvr\n3\n, and P-element excision lines qvr\nip6\n(imprecise jumpout), qvr\nrv7\n, and qvr\nrv9\n(revertants) derived from qvr\nEY\n. Distinct from the original intron-located qvr\n1\nallele that generates abnormal-sized mRNAs, qvr\n2\n, and qvr\n3\nhad their lesion sites in exons 6 and 7, respectively, producing nearly normal-sized mRNA products. A set of RNA-editing sites are nearby the lesion sites of qvr\n3\nand qvr\nEY\non exon 7. Except for the revertants, all qvr alleles display a clear ether-induced leg-shaking phenotype just like Sh, and weakened climbing abilities to varying degrees. Unlike Sh, all shaking qvr alleles (except for qvr\nf01257\n) displayed a unique activity-dependent enhancement in excitatory junction potentials (EJPs) at larval neuromuscular junctions (NMJs) at very low stimulus frequencies, with qvr\nEY\ndisplaying the largest EJP and more significant NMJ overgrowth than other alleles. Our detailed characterization of a collection of qvr alleles helps to establish links between novel molecular lesions and different behavioral and physiological consequences, revealing how modifications of the qvr gene lead to a wide spectrum of phenotypes, including neuromuscular hyperexcitability, defective motor ability and activity-rest cycles.
Details
- Title: Subtitle
- Generation and characterization of new alleles of quiver (qvr) that encodes an extracellular modulator of the Shaker potassium channel
- Creators
- Hongyu Ruan - Department of Biology, University of IowaAtsushi Ueda - Department of Biology, University of IowaXiaomin Xing - Department of Biology, University of IowaXuxuan Wan - Department of Biology, University of IowaBenjamin Strub - Department of Biology, York UniversitySpencer Mukai - Department of Biology, York UniversityKaan Certel - Department of Molecular Physiology and Biophysics, University of IowaDavid Green - Department of Biology, University of IowaKyle Belozerov - Department of Biology, York UniversityWei-Dong Yao - Department of Biology, University of IowaWayne Johnson - Department of Molecular Physiology and Biophysics, University of IowaJim Jung-Ching Lin - Department of Biology, University of IowaArthur J Hilliker - Department of Biology, York UniversityChun-Fang Wu - Department of Biology, University of Iowa
- Resource Type
- Journal article
- Publication Details
- Journal of neurogenetics, Vol.31(4), pp.325-336
- DOI
- 10.1080/01677063.2017.1393076
- PMID
- 29117754
- PMCID
- PMC5918287
- NLM abbreviation
- J Neurogenet
- ISSN
- 0167-7063
- eISSN
- 1563-5260
- Publisher
- Taylor & Francis
- Grant note
- NS26528; GM80255; AG047612; AG051513 / NIH\nNatural Sciences and Engineering Research Council
- Language
- English
- Date published
- 10/02/2017
- Academic Unit
- Neurology; Molecular Physiology and Biophysics; Iowa Neuroscience Institute; Biology
- Record Identifier
- 9984070334302771
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