Generation and maintenance of Listeria-specific CD8 + T cell responses in perforin-deficient mice chronically infected with LCMV

Troy D Humphreys; Aaruni Khanolkar; Vladimir P Badovinac; John T Harty

doi:10.1016/j.virol.2007.08.038

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Generation and maintenance of Listeria-specific CD8 + T cell responses in perforin-deficient mice chronically infected with LCMV

Journal article

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Generation and maintenance of Listeria-specific CD8 + T cell responses in perforin-deficient mice chronically infected with LCMV

Troy D Humphreys, Aaruni Khanolkar, Vladimir P Badovinac and John T Harty

Virology (New York, N.Y.), Vol.370(2), pp.310-322

2008

DOI: 10.1016/j.virol.2007.08.038

PMCID: PMC2214839

PMID: 17936870

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Abstract

Disruption of the perforin gene results in primary immunodeficiency and an increased susceptibility to opportunistic pathogens. Perforin-deficient (PKO) mice fail to clear primary lymphocytic choriomeningitis virus (LCMV) Armstrong, resulting in persistent infection and functional exhaustion of virus-specific CD8 + T cells. CD8 + T cell responses to Listeria monocytogenes (LM) challenge within the first week after LCMV infection were diminished in both WT and PKO mice, and correlated with enhanced bacterial clearance. However, bacterial challenge at later time points generated similar CD8 T cell responses in both groups of mice. The phenotype and function of pre-existing LM-specific memory CD8 + T cells were maintained in persistently infected PKO mice. Thus persistent LCMV infection, as a result of perforin deficiency, results in dysfunction of the virus-specific CD8 + T cell response but does not compromise the host's ability to maintain pre-existing memory CD8 + T cells or to generate new memory CD8 + T cell responses against other pathogens.

Lymphocytic choriomeningitis virus

Memory

Immunodeficiency

Listeria monocytogenes

Perforin

Immunity

CD8 T cells

Details

Title: Subtitle: Generation and maintenance of Listeria-specific CD8 + T cell responses in perforin-deficient mice chronically infected with LCMV
Creators: Troy D Humphreys - Department of Microbiology, University of Iowa, Iowa City, IA 52242, USA
Aaruni Khanolkar - Department of Microbiology, University of Iowa, Iowa City, IA 52242, USA
Vladimir P Badovinac - Department of Microbiology, University of Iowa, Iowa City, IA 52242, USA
John T Harty - Department of Microbiology, University of Iowa, Iowa City, IA 52242, USA
Resource Type: Journal article
Publication Details: Virology (New York, N.Y.), Vol.370(2), pp.310-322
DOI: 10.1016/j.virol.2007.08.038
PMID: 17936870
PMCID: PMC2214839
NLM abbreviation: Virology
ISSN: 0042-6822
eISSN: 1096-0341
Publisher: Elsevier Inc
Language: English
Date published: 2008
Academic Unit: Microbiology and Immunology; Pathology
Record Identifier: 9984047755402771

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