Logo image
Back
Generation and validation of a D1 dopamine receptor Flpo knock-in mouse
Journal article   Peer reviewed

Generation and validation of a D1 dopamine receptor Flpo knock-in mouse

Alexis M Oppman, William J Paradee, Nandakumar S Narayanan and Young-Cho Kim
Journal of neuroscience methods, Vol.415, 110345
03/2025
DOI: 10.1016/j.jneumeth.2024.110345
PMCID: PMC11878109
PMID: 39701542
url
https://pmc.ncbi.nlm.nih.gov/articles/PMC11878109/pdf/nihms-2049723.pdfView
Open Access

Abstract

Dopamine is a powerful neuromodulator of diverse brain functions, including movement, motivation, reward, and cognition. D1-type dopamine receptors (D1DRs) are the most prevalently expressed dopamine receptors in the brain. Neurons expressing D1DRs are heterogeneous and involve several subpopulations. Although these neurons can be studied with BAC-transgenic rodents, these models have some limitations especially when considering their integration with conditional or intersectional genetic tools. We developed a novel Drd1-P2A-Flpo (Drd1-Flpo) mouse line in which the Flpo gene was knocked in immediately after the Drd1 gene using CRISPR-Cas9. We validated the Drd1-Flpo line by confirming Flp expression and functionality specific to D1DR+ neurons with immunohistochemistry and in situ hybridization. The Drd1-Flpo line is a useful resource for studying subpopulations of D1DR+ neurons with intersectional genetic tools. We demonstrated brain-wide GFP expression driven by Drd1-Flpo, suggesting that this mouse line may be useful for comprehensive anatomical and functional studies in many brain regions. The Drd1-Flpo model will advance the study of dopaminergic signaling by providing a new tool for investigating the diverse roles of D1DR+ neurons and their subpopulations in brain disease.
 intersectional genetics D1 dopamine receptor D1DR transgenic animal CRISPR-Cas9 Flpo recombinase

Details

Metrics

10 Record Views
Logo image