Generation of PDGFRα + Cardioblasts from Pluripotent Stem Cells

Seon Pyo Hong; Sukhyun Song; Sung Woo Cho; Seungjoo Lee; Bong Ihn Koh; Hosung Bae; Kyun Hoo Kim; Jin-Sung Park; Hyo-Sang Do; Ilkyun Im; Hye Jin Heo; Tae Hee Ko; Jae-Hyeong Park; Jae Boum Youm; Seong-Jin Kim; Injune Kim; Jin Han; Yong-Mahn Han; Gou Young Koh

doi:10.1038/srep41840

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Generation of PDGFRα + Cardioblasts from Pluripotent Stem Cells

Journal article

Open access

Peer reviewed

Generation of PDGFRα + Cardioblasts from Pluripotent Stem Cells

Seon Pyo Hong, Sukhyun Song, Sung Woo Cho, Seungjoo Lee, Bong Ihn Koh, Hosung Bae, Kyun Hoo Kim, Jin-Sung Park, Hyo-Sang Do, Ilkyun Im, …

Scientific reports, Vol.7(1), pp.41840-41840

02/06/2017

DOI: 10.1038/srep41840

PMID: 28165490

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Abstract

Isolating actively proliferating cardioblasts is the first crucial step for cardiac regeneration through cell implantation. However, the origin and identity of putative cardioblasts are still unclear. Here, we uncover a novel class of cardiac lineage cells, PDGFRα Flk1 cardioblasts (PCBs), from mouse and human pluripotent stem cells induced using CsAYTE, a combination of the small molecules Cyclosporin A, the rho-associated coiled-coil kinase inhibitor Y27632, the antioxidant Trolox, and the ALK5 inhibitor EW7197. This novel population of actively proliferating cells is cardiac lineage-committed but in a morphologically and functionally immature state compared to mature cardiomyocytes. Most important, most of CsAYTE-induced PCBs spontaneously differentiated into functional αMHC cardiomyocytes (M CMs) and could be a potential cellular resource for cardiac regeneration.

Amides - pharmacology

Aniline Compounds - pharmacology

Animals

Antioxidants - pharmacology

Cell Differentiation

Cell Line

Cells, Cultured

Chromans - pharmacology

Cyclosporine - pharmacology

Enzyme Inhibitors - pharmacology

Humans

Mice

Myoblasts - cytology

Myoblasts - metabolism

Myocytes, Cardiac - cytology

Myocytes, Cardiac - metabolism

Pluripotent Stem Cells - cytology

Pluripotent Stem Cells - drug effects

Pluripotent Stem Cells - metabolism

Pyridines - pharmacology

Receptor, Platelet-Derived Growth Factor alpha - genetics

Receptor, Platelet-Derived Growth Factor alpha - metabolism

Triazoles - pharmacology

Details

Title: Subtitle: Generation of PDGFRα + Cardioblasts from Pluripotent Stem Cells
Creators: Seon Pyo Hong - Korea Advanced Institute of Science and Technology
Sukhyun Song - Institute for Basic Science
Sung Woo Cho - Korea Advanced Institute of Science and Technology
Seungjoo Lee - Korea Advanced Institute of Science and Technology
Bong Ihn Koh - Korea Advanced Institute of Science and Technology
Hosung Bae - Korea Advanced Institute of Science and Technology
Kyun Hoo Kim - Korea Advanced Institute of Science and Technology
Jin-Sung Park - Institute for Basic Science
Hyo-Sang Do - Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, Korea
Ilkyun Im - Korea Advanced Institute of Science and Technology
Hye Jin Heo - Inje University
Tae Hee Ko - Inje University
Jae-Hyeong Park - Inje University
Jae Boum Youm - Inje University
Seong-Jin Kim - CHA University
Injune Kim - Korea Advanced Institute of Science and Technology
Jin Han - Inje University
Yong-Mahn Han - Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, Korea
Gou Young Koh - Korea Advanced Institute of Science and Technology
Resource Type: Journal article
Publication Details: Scientific reports, Vol.7(1), pp.41840-41840
DOI: 10.1038/srep41840
PMID: 28165490
NLM abbreviation: Sci Rep
ISSN: 2045-2322
eISSN: 2045-2322
Language: English
Date published: 02/06/2017
Academic Unit: Internal Medicine
Record Identifier: 9985217030202771

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