Journal article
Generation of a Novel Transgenic Mouse Model for Bioluminescent Monitoring of Survivin Gene Activity in Vivo at Various Pathophysiological Processes
The American journal of pathology, Vol.176(4), pp.1629-1638
2010
DOI: 10.2353/ajpath.2010.090414
PMCID: PMC2843455
PMID: 20133811
Abstract
Survival has been implicated to play an important role in various pathophysiological processes. However, because of a lack of appropriate animal models, the role and dynamic expression of survivin during pathophysiology are not well defined. We generated a human survivin gene promoter-driven luciferase transgenic mouse model (SPlucTg) so that dynamic survivin gene activity can be monitored during various pathophysiological conditions using
in vivo
imaging. Our results show that, consistent with survivin positivity in testis, luciferase activity in normal SPlucTg mice was detected in the testis of male mice. Furthermore, similar to the known requirement of transient expression of survivin for pathophysiological responses, we observed a transient luciferase expression in castrated SPlucTg male mice after supplement of androgen. Significantly, it was reported that survivin expression turns on during mouse liver injury and regeneration; a transient and dose-dependent luciferase expression in the mouse liver was observed after administration of carbon tetrachloride into SPlucTg mice. We further demonstrated that luciferase activity closely correlates with endogenous survivin expression. We also demonstrated that only a subset of cells expresses survivin, and its expression overlaps with the expression of several stem cell markers tested. Thus, we have generated a unique animal model for analysis of diverse pathophysiological processes and possible stem cell distribution/activity
in vivo
.
Details
- Title: Subtitle
- Generation of a Novel Transgenic Mouse Model for Bioluminescent Monitoring of Survivin Gene Activity in Vivo at Various Pathophysiological Processes
- Creators
- Fengzhi Li - Roswell Park Cancer InstituteQiuying Cheng - Roswell Park Cancer InstituteXiang Ling - Roswell Park Cancer InstituteAimee Stablewski - Roswell Park Cancer InstituteLei Tang - Roswell Park Cancer InstituteBarbara A. Foster - Roswell Park Cancer InstituteCandace S. Johnson - Roswell Park Cancer InstituteYoucef M. Rustum - Roswell Park Cancer InstituteCarl W. Porter - Roswell Park Cancer Institute
- Resource Type
- Journal article
- Publication Details
- The American journal of pathology, Vol.176(4), pp.1629-1638
- Publisher
- Elsevier Inc
- DOI
- 10.2353/ajpath.2010.090414
- PMID
- 20133811
- PMCID
- PMC2843455
- ISSN
- 0002-9440
- eISSN
- 1525-2191
- Language
- English
- Date published
- 2010
- Academic Unit
- Hematology, Oncology, and Blood & Marrow Transplantation; Internal Medicine
- Record Identifier
- 9984359763302771
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