Journal article
Generation of a multipurpose Prdm16 mouse allele by targeted gene trapping
Disease models & mechanisms, Vol.10(7), pp.909-922
07/01/2017
DOI: 10.1242/dmm.029561
PMCID: PMC5536910
PMID: 28424158
Abstract
Gene trap mutagenesis is a powerful tool to create loss-of-function mutations in mice and other model organisms. Modifications of traditional gene trap cassettes, including addition of conditional features in the form of Flip-excision (FlEx) arrays to enable directional gene trap cassette inversions by Cre and Flpe site-specific recombinases, greatly enhanced their experimental potential. By taking advantage of these conditional gene trap cassettes, we developed a generic strategy for generating conditional mutations and validated this strategy in mice carrying a multipurpose allele of the Prdm16 transcription factor gene. We demonstrate that the gene trap insertion creates a null mutation replicating the Pierre Robin sequence-type cleft palate phenotype of other Prdm16 mutant mice. Consecutive breeding to Flpe and Emx1(IREScre) deleter mice spatially restricted Prdm16 loss to regions of the forebrain expressing the homeobox gene Emx1, demonstrating the utility of the technology for the analysis of tissue-specific gene functions.
Details
- Title: Subtitle
- Generation of a multipurpose Prdm16 mouse allele by targeted gene trapping
- Creators
- Alexander Strassman - Chicago College of Osteopathic MedicineFrank Schnuetgen - Goethe Univ, Univ Hosp Frankfurt, Dept Mol Hematol, D-60590 Frankfurt, GermanyQi Dai - Stockholm UniversityJennifer C. Jones - Chicago College of Osteopathic MedicineAngela C. Gomez - Chicago College of Osteopathic MedicineLenore Pitstick - Chicago College of Osteopathic MedicineNathan E. Holton - University of IowaRussell Moskal - Chicago College of Osteopathic MedicineErin R. Leslie - Chicago College of Osteopathic MedicineHarald von Melchner - University Hospital FrankfurtDavid R. Beier - University of WashingtonBryan C. Bjork - Chicago College of Osteopathic Medicine
- Resource Type
- Journal article
- Publication Details
- Disease models & mechanisms, Vol.10(7), pp.909-922
- DOI
- 10.1242/dmm.029561
- PMID
- 28424158
- PMCID
- PMC5536910
- NLM abbreviation
- Dis Model Mech
- ISSN
- 1754-8403
- eISSN
- 1754-8411
- Publisher
- COMPANY OF BIOLOGISTS LTD
- Number of pages
- 14
- Grant note
- R01MH081187 / NATIONAL INSTITUTE OF MENTAL HEALTH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Mental Health (NIMH) NGFNplus-DiGtoPconsortium/01GS0858 / German Ministry of Education and Science R15DE023982 / NATIONAL INSTITUTE OF DENTAL & CRANIOFACIAL RESEARCH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Dental & Craniofacial Research (NIDCR) K12 DE014528; R15 DE023982; R01 DE015246; R01 HD036404 / National Institutes of Health (NIDCR); United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Dental & Craniofacial Research (NIDCR) R01HD036404 / EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH & HUMAN DEVELOPMENT; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD) ME 820/6-1 / Deutsche Forschungsgemeinschaft; German Research Foundation (DFG) K12DE014528 / NATIONAL INSTITUTE OF DENTAL &CRANIOFACIAL RESEARCH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Dental & Craniofacial Research (NIDCR) National Institutes of Health (NICHD); United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
- Language
- English
- Date published
- 07/01/2017
- Academic Unit
- Anatomy and Cell Biology; Craniofacial Anomalies Research Center
- Record Identifier
- 9984284358502771
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