Journal article
Generation of cAMP-activated chloride currents by expression of CFTR
Science (American Association for the Advancement of Science), Vol.251(4994), pp.679-682
02/08/1991
DOI: 10.1126/science.1704151
PMID: 1704151
Abstract
Mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) cause cystic fibrosis. In order to evaluate its function, CFTR was expressed in HeLa, Chinese hamster ovary (CHO), and NIH 3T3 fibroblast cells, and anion permeability was assessed with a fluorescence microscopic assay and the whole-cell patch-clamp technique. Adenosine 3',5'-monophosphate (cAMP) increased anion permeability and chloride currents in cells expressing CFTR, but not in cells expressing a mutant CFTR (delta F508) or in nontransfected cells. The simplest interpretation of these observations is that CFTR is itself a cAMP-activated chloride channel. The alternative interpretation, that CFTR directly or indirectly regulates chloride channels, requires that these cells have endogenous cryptic, chloride channels that are stimulated by cAMP only in the presence of CFTR.
Details
- Title: Subtitle
- Generation of cAMP-activated chloride currents by expression of CFTR
- Creators
- M P Anderson - Department of Internal Medicine, University of Iowa College of Medicine, Iowa City 52242D P RichR J GregoryA E SmithM J Welsh
- Resource Type
- Journal article
- Publication Details
- Science (American Association for the Advancement of Science), Vol.251(4994), pp.679-682
- Publisher
- United States
- DOI
- 10.1126/science.1704151
- PMID
- 1704151
- ISSN
- 0036-8075
- eISSN
- 1095-9203
- Language
- English
- Date published
- 02/08/1991
- Academic Unit
- Neurology; Molecular Physiology and Biophysics; Neurosurgery; Internal Medicine
- Record Identifier
- 9984020729602771
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