Journal article
Generation of cytotoxic T lymphocytes against modified self in the absence of antigen by interleukin 2-containing preparations
Cellular immunology, Vol.76(1), pp.81-93
1983
DOI: 10.1016/0008-8749(83)90350-7
PMID: 6187486
Abstract
Spleen cells obtained from normal mice were cultured with interleukin 2 (IL2); no antigen was added. After 4–5 days, these cultures contained effector cells which lysed autologous spleen target cells that were modified with 2,4,6-trinitrobenzene sulfonic acid or fluorescein isothiocyanate. No killing was seen on unmodified spleen target cells. These effector cells were Thy 1
+, Lyt 1
−, 2
+ and were derived from Thy 1
+ precursor cells. IL2 preparations induced the generation of such cytotoxic T lymphocytes (CTL) in a dose-dependent manner. IL2-induced CTL were shown to be different from the natural killer (NK) cells augmented by IL2 by virtue of their time of appearance in culture, by cold-target competition, and by different cell-surface markers. These results demonstrate that the IL2 signal may be sufficient for the induction of the differentiation of CTL precursors in the absence of an antigenic signal.
Details
- Title: Subtitle
- Generation of cytotoxic T lymphocytes against modified self in the absence of antigen by interleukin 2-containing preparations
- Creators
- Zuhair K BallasGerald B Ahmann
- Resource Type
- Journal article
- Publication Details
- Cellular immunology, Vol.76(1), pp.81-93
- Publisher
- Elsevier Inc
- DOI
- 10.1016/0008-8749(83)90350-7
- PMID
- 6187486
- ISSN
- 0008-8749
- eISSN
- 1090-2163
- Language
- English
- Date published
- 1983
- Academic Unit
- Immunology; Internal Medicine
- Record Identifier
- 9984094589902771
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