Journal article
Genetic Requirements for RAD51- and RAD54-Independent Break-Induced Replication Repair of a Chromosomal Double-Strand Break
Molecular and cellular biology, Vol.21(6), pp.2048-2056
03/2001
DOI: 10.1128/MCB.21.6.2048-2056.2001
PMCID: PMC86809
PMID: 11238940
Abstract
Broken chromosomes can be repaired by several homologous recombination mechanisms, including gene conversion and break-induced replication (BIR). In Saccharomyces cerevisiae, an HO endonuclease-induced double-strand break (DSB) is normally repaired by gene conversion. Previously, we have shown that in the absence ofRAD52, repair is nearly absent and diploid cells lose the broken chromosome; however, in cells lacking RAD51, gene conversion is absent but cells can repair the DSB by BIR. We now report that gene conversion is also abolished when RAD54, RAD55, and RAD57 are deleted but BIR occurs, as withrad51Δ cells. DSB-induced gene conversion is not significantly affected when RAD50, RAD59, TID1(RDH54), SRS2, or SGS1 is deleted. Various double mutations largely eliminate both gene conversion and BIR, including rad51Δ rad50Δ, rad51Δ rad59Δ, andrad54Δ tid1Δ. These results demonstrate that there is aRAD51- and RAD54-independent BIR pathway that requires RAD59, TID1, RAD50, and presumablyMRE11 and XRS2. The similar genetic requirements for BIR and telomere maintenance in the absence of telomerase also suggest that these two processes proceed by similar mechanisms.
Details
- Title: Subtitle
- Genetic Requirements for RAD51- and RAD54-Independent Break-Induced Replication Repair of a Chromosomal Double-Strand Break
- Creators
- Laurence Signon - Department of Biology and Rosenstiel Center, Brandeis University, Waltham, Massachusetts 02254-9110, and Department of Biochemistry, New York University School of Medicine, New York, New York 10016Anna Malkova - Department of Biology and Rosenstiel Center, Brandeis University, Waltham, Massachusetts 02254-9110, and Department of Biochemistry, New York University School of Medicine, New York, New York 10016Maria L Naylor - Department of Biology and Rosenstiel Center, Brandeis University, Waltham, Massachusetts 02254-9110, and Department of Biochemistry, New York University School of Medicine, New York, New York 10016Hannah Klein - Department of Biology and Rosenstiel Center, Brandeis University, Waltham, Massachusetts 02254-9110, and Department of Biochemistry, New York University School of Medicine, New York, New York 10016James E Haber - Department of Biology and Rosenstiel Center, Brandeis University, Waltham, Massachusetts 02254-9110, and Department of Biochemistry, New York University School of Medicine, New York, New York 10016
- Resource Type
- Journal article
- Publication Details
- Molecular and cellular biology, Vol.21(6), pp.2048-2056
- DOI
- 10.1128/MCB.21.6.2048-2056.2001
- PMID
- 11238940
- PMCID
- PMC86809
- NLM abbreviation
- Mol Cell Biol
- ISSN
- 0270-7306
- eISSN
- 1098-5549
- Publisher
- American Society for Microbiology
- Language
- English
- Date published
- 03/2001
- Academic Unit
- Biology
- Record Identifier
- 9984217423802771
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