Journal article
Genetic Variation among Panton-Valentine Leukocidin-Encoding Bacteriophages in Staphylococcus aureus Clonal Complex 30 Strains
Journal of clinical microbiology, Vol.51(3), pp.914-919
03/2013
DOI: 10.1128/JCM.03015-12
PMCID: PMC3592069
PMID: 23284024
Abstract
Clonal complex 30 (CC30), one of the major
Staphylococcus aureus
lineages, has caused extensive hospital-acquired and community-acquired infections worldwide. Recent comparative genomics studies have demonstrated that three CC30 clones—phage type 80/81, Southwest Pacific (SWP), and contemporary EMRSA-16 associated (Con) strains—shared a recent common ancestor more than 100 years ago. Panton-Valentine leukocidin (PVL), a bacteriophage encoded toxin that has been epidemiologically linked with community-associated methicillin-resistant
S. aureus
(CA-MRSA), has frequently been identified in CC30 clones, although the
pvl
gene variation and distribution of PVL-encoding phages are poorly understood. We determined here the distribution of PVL phages, PVL gene sequences, and chromosomal phage insertion sites in 52
S. aureus
CC30 PVL-harboring isolates, collected from four continents over a 75-year period. Our results indicate that PVL phages with icosahedral heads, including Φ108PVL and ΦPVL, were mainly associated with phage 80/81 strains, whereas phages with elongated heads were predominantly found in SWP (ΦSa2958 and ΦTCH60) and Con (ΦSa2USA) strains. Nine single-nucleotide polymorphisms were identified in the
lukSF-PV
gene, with six isolates harboring the R variant that has been previously associated with CA-MRSA strains. Interestingly, all six R variant strains belonged to the same Con CC30 clone and carried a ΦSa2USA-like phage. Similar chromosomal phage insertion sites were also identified in all 52 PVL-harboring CC30 strains. These analyses provide important insights into the microepidemiology of PVL-harboring CC30 strains, while the discovery of ΦSa2USA-associated R variant strains sheds further light on the evolution of PVL-positive CA-MRSA.
Details
- Title: Subtitle
- Genetic Variation among Panton-Valentine Leukocidin-Encoding Bacteriophages in Staphylococcus aureus Clonal Complex 30 Strains
- Creators
- Liang Chen - Public Health Research Institute Center, International Center for Public Health, University of Medicine and Dentistry of New Jersey, Newark, New Jersey, USAKalyan D Chavda - Public Health Research Institute Center, International Center for Public Health, University of Medicine and Dentistry of New Jersey, Newark, New Jersey, USAMihir Solanki - Public Health Research Institute Center, International Center for Public Health, University of Medicine and Dentistry of New Jersey, Newark, New Jersey, USAJosé R Mediavilla - Public Health Research Institute Center, International Center for Public Health, University of Medicine and Dentistry of New Jersey, Newark, New Jersey, USABarun Mathema - Public Health Research Institute Center, International Center for Public Health, University of Medicine and Dentistry of New Jersey, Newark, New Jersey, USAPatrick M Schlievert - Department of Microbiology, Carver College of Medicine, Iowa City, Iowa, USABarry N Kreiswirth - Public Health Research Institute Center, International Center for Public Health, University of Medicine and Dentistry of New Jersey, Newark, New Jersey, USA
- Resource Type
- Journal article
- Publication Details
- Journal of clinical microbiology, Vol.51(3), pp.914-919
- Publisher
- American Society for Microbiology; 1752 N St., N.W., Washington, DC
- DOI
- 10.1128/JCM.03015-12
- PMID
- 23284024
- PMCID
- PMC3592069
- ISSN
- 0095-1137
- eISSN
- 1098-660X
- Language
- English
- Date published
- 03/2013
- Academic Unit
- Microbiology and Immunology; Internal Medicine
- Record Identifier
- 9984001134902771
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