Genetic and developmental analysis of polytene section 17 of the X chromosome of Drosophila melanogaster

D. F EBERL; L. A PERKINS; M ENGELSTEIN; A. J HILLIKER; N PERRIMON

doi:10.1093/genetics/130.3.569

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Genetic and developmental analysis of polytene section 17 of the X chromosome of Drosophila melanogaster

Journal article

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Genetic and developmental analysis of polytene section 17 of the X chromosome of Drosophila melanogaster

D. F EBERL, L. A PERKINS, M ENGELSTEIN, A. J HILLIKER and N PERRIMON

Genetics (Austin), Vol.130(3), pp.569-583

1992

DOI: 10.1093/genetics/130.3.569

PMCID: PMC1204874

PMID: 1551578

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Abstract

Polytene section 17 of the X chromosome of Drosophila melanogaster, previously known to contain six putative lethal complementation groups important in oogenesis and embryogenesis, has here been further characterized genetically and developmentally. We constructed fcl+Y, a duplication of this region, which allowed us to conduct mutagenesis screens specific for the region and to perform complementation analyses (previously not possible). We recovered 67 new lethal mutations which defined 15 complementation groups within Df(1)N19 which deletes most of polytene section 17. The zygotic lethal phenotypes of these and preexisting mutations within polytene section 17 were examined, and their maternal requirements were analysed in homozygous germline clones using the dominant female sterile technique. We present evidence that an additional gene, which produces two developmentally regulated transcripts, is located in this region and is involved in embryogenesis, although no mutations in this gene were identified. In this interval of 37 to 43 polytene chromosome bands we have defined 17 genes, 12 (71%) of which are of significance to oogenesis or embryogenesis.

Fundamental and applied biological sciences. Psychology

Classical genetics, quantitative genetics, hybrids

Biological and medical sciences

Animals

Genetics of eukaryotes. Biological and molecular evolution

Invertebrata

Details

Title: Subtitle: Genetic and developmental analysis of polytene section 17 of the X chromosome of Drosophila melanogaster
Creators: D. F EBERL - Univ. Guelph, dep. molecular biology genetics, Guelph ON N1G 2W1, Canada
L. A PERKINS - Univ. Guelph, dep. molecular biology genetics, Guelph ON N1G 2W1, Canada
M ENGELSTEIN - Univ. Guelph, dep. molecular biology genetics, Guelph ON N1G 2W1, Canada
A. J HILLIKER - Univ. Guelph, dep. molecular biology genetics, Guelph ON N1G 2W1, Canada
N PERRIMON - Univ. Guelph, dep. molecular biology genetics, Guelph ON N1G 2W1, Canada
Resource Type: Journal article
Publication Details: Genetics (Austin), Vol.130(3), pp.569-583
Publisher: Genetics Society of America; Bethesda, MD
DOI: 10.1093/genetics/130.3.569
PMID: 1551578
PMCID: PMC1204874
ISSN: 0016-6731
eISSN: 1943-2631
Language: English
Date published: 1992
Academic Unit: Iowa Neuroscience Institute; Biology
Record Identifier: 9984071689902771

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