Genetic and molecular characterization of P element-induced mutations reveals that the Drosophila ovarian tumor gene has maternal activity and a variable null phenotype

Pamela K Geyer; J Scott Patton; Christopher Rodesch; Rod N Nagoshi

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Genetic and molecular characterization of P element-induced mutations reveals that the Drosophila ovarian tumor gene has maternal activity and a variable null phenotype

Journal article

Peer reviewed

Genetic and molecular characterization of P element-induced mutations reveals that the Drosophila ovarian tumor gene has maternal activity and a variable null phenotype

Pamela K Geyer, J Scott Patton, Christopher Rodesch and Rod N Nagoshi

Genetics (Austin), Vol.133(2), pp.265-278

1993

PMCID: PMC1205317

PMID: 8436275

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Abstract

The mutations in the ovarian tumor (otu) gene arrest oogenesis at several stages in development. A series of deletion mutations in the otu region were characterized, each of which causes the absence or reduction of the otu transcript. These alleles range from the most severe class, which results in ovaries lacking egg cysts, to relatively mild mutations that allow the development of late stage oocytes. Heteroallelic combinations of these mutations demonstrate that the phenotypic complexity of otu mutant ovaries is due to a dosage dependent requirement for otu activity. Reciprocal cross and developmental Northern blot studies suggest a maternal requirement for otu in the development of the female germline. In addition we demonstrate that the otu zygotic null phenotype is variable, ranging from the absence of cysts in the most extreme cases, to the presence of tumorous egg chambers.

Fundamental and applied biological sciences. Psychology

Classical genetics, quantitative genetics, hybrids

Biological and medical sciences

Animals

Genetics of eukaryotes. Biological and molecular evolution

Invertebrata

Details

Title: Subtitle: Genetic and molecular characterization of P element-induced mutations reveals that the Drosophila ovarian tumor gene has maternal activity and a variable null phenotype
Creators: Pamela K Geyer - Univ. Iowa, dep. biochemistry, Iowa City IA 52242, United States
J Scott Patton - Univ. Iowa, dep. biochemistry, Iowa City IA 52242, United States
Christopher Rodesch - Univ. Iowa, dep. biochemistry, Iowa City IA 52242, United States
Rod N Nagoshi - Univ. Iowa, dep. biochemistry, Iowa City IA 52242, United States
Resource Type: Journal article
Publication Details: Genetics (Austin), Vol.133(2), pp.265-278
Publisher: Genetics Society of America; Bethesda, MD
PMID: 8436275
PMCID: PMC1205317
ISSN: 0016-6731
eISSN: 1943-2631
Language: English
Date published: 1993
Academic Unit: Obstetrics and Gynecology; Biochemistry and Molecular Biology
Record Identifier: 9984025400002771

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