Genetic engineering of microalgae for enhanced lipid production

Camilo F. Muñoz; Christian Südfeld; Mihris I.S. Naduthodi; Ruud A. Weusthuis; Maria J. Barbosa; René H. Wijffels; Sarah D’Adamo

doi:10.1016/j.biotechadv.2021.107836

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Genetic engineering of microalgae for enhanced lipid production

Journal article

Peer reviewed

Genetic engineering of microalgae for enhanced lipid production

Camilo F. Muñoz, Christian Südfeld, Mihris I.S. Naduthodi, Ruud A. Weusthuis, Maria J. Barbosa, René H. Wijffels and Sarah D’Adamo

Biotechnology advances, Vol.52, pp.107836-107836

11/15/2021

DOI: 10.1016/j.biotechadv.2021.107836

PMID: 34534633

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https://research.wur.nl/en/publications/genetic-engineering-of-microalgae-for-enhanced-lipid-productionView

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Abstract

Microalgae have the potential to become microbial cell factories for lipid production. Their ability to convert sunlight and CO2 into valuable lipid compounds has attracted interest from cosmetic, biofuel, food and feed industries. In order to make microalgae-derived products cost-effective and commercially competitive, enhanced growth rates and lipid productivities are needed, which require optimization of cultivation systems and strain improvement. Advances in genetic tool development and omics technologies have increased our understanding of lipid metabolism, which has opened up possibilities for targeted metabolic engineering. In this review we provide a comprehensive overview on the developments made to genetically engineer microalgal strains over the last 30 years. We focus on the strategies that lead to an increased lipid content and altered fatty acid profile. These include the genetic engineering of the fatty acid synthesis pathway, Kennedy pathway, polyunsaturated fatty acid and triacylglycerol metabolisms and fatty acid catabolism. Moreover, genetic engineering of specific transcription factors, NADPH generation and central carbon metabolism, which lead to increase of lipid accumulation are also reviewed. •30 years of genetic engineering for increasing microalgal lipid content is reviewed.•Strategies used to enhance PUFAs and TAGs production are provided.•Engineering of NADPH and central metabolism, TFs and FA catabolism are also revised.

Fatty acids

Gene editing

Genetic engineering

Heterologous expression

Microalgae

Omics

Overexpression

Polyunsaturated fatty acids (PUFA)

Triacylglycerols (TAG)

Details

Title: Subtitle: Genetic engineering of microalgae for enhanced lipid production
Creators: Camilo F. Muñoz - Wageningen University & Research
Christian Südfeld - Wageningen University & Research
Mihris I.S. Naduthodi - Wageningen University & Research
Ruud A. Weusthuis - Wageningen University & Research
Maria J. Barbosa - Bioprocess Engineering, AlgaePARC, Wageningen University and Research, PO Box 16, 6700 AA Wageningen, The Netherlands
René H. Wijffels - Wageningen University & Research
Sarah D’Adamo - Bioprocess Engineering, AlgaePARC, Wageningen University and Research, PO Box 16, 6700 AA Wageningen, The Netherlands
Resource Type: Journal article
Publication Details: Biotechnology advances, Vol.52, pp.107836-107836
DOI: 10.1016/j.biotechadv.2021.107836
PMID: 34534633
NLM abbreviation: Biotechnol Adv
ISSN: 0734-9750
eISSN: 1873-1899
Publisher: Elsevier Inc
Language: English
Date published: 11/15/2021
Academic Unit: International Programs; Spanish and Portuguese
Record Identifier: 9984398033802771

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