Genetic instability in Drosophila melanogaster: P-element mutagenesis by gene conversion

Pamela K Geyer; Karen L Richardson; Victor G Corces; M M Green

doi:10.1073/pnas.85.17.6455

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Genetic instability in Drosophila melanogaster: P-element mutagenesis by gene conversion

Journal article

Open access

Peer reviewed

Genetic instability in Drosophila melanogaster: P-element mutagenesis by gene conversion

Pamela K Geyer, Karen L Richardson, Victor G Corces and M M Green

Proceedings of the National Academy of Sciences - PNAS, Vol.85(17), pp.6455-6459

09/1988

DOI: 10.1073/pnas.85.17.6455

PMCID: PMC281991

PMID: 2842772

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Abstract

We report the molecular characterization of several P element-induced mutations and their revertants at the yellow (y) locus of Drosophila melanogaster. One of the mutants analyzed, y76d28, results from the insertion of a P element into the 5'-transcribed, untranslated portion of the y gene. Sequence analysis of several revertants of y76d28 shows that P excision occurs imprecisely. These events result in insertion of additional ATG codons in the y locus mRNA but are without phenotypic effect. In addition, we describe the molecular structure of P-associated mutations induced in a near wild-type revertant of y76d28 that carries an internally deleted 0.4-kilobase P element in the 5' noncoding region. Sequence analysis of two of these mutants demonstrates that they arose as a result of the integration of a larger P element at the exact location as in the parental stock without the 8-base-pair additional duplication associated with P insertions. The phenotype of these y alleles is dependent on the size and orientation of the integrated P element. We infer that P-element replacement in these mutants has occurred by a recombination/gene conversion mechanism.

Mutation

Animals

DNA Restriction Enzymes

Base Sequence

Alleles

Molecular Sequence Data

Transcription, Genetic

Nucleotide Mapping

Drosophila melanogaster - genetics

DNA Transposable Elements

Gene Conversion

Details

Title: Subtitle: Genetic instability in Drosophila melanogaster: P-element mutagenesis by gene conversion
Creators: Pamela K Geyer - Department of Biology, Johns Hopkins University, Baltimore, MD 21218
Karen L Richardson
Victor G Corces
M M Green - University of California, Davis
Resource Type: Journal article
Publication Details: Proceedings of the National Academy of Sciences - PNAS, Vol.85(17), pp.6455-6459
DOI: 10.1073/pnas.85.17.6455
PMID: 2842772
PMCID: PMC281991
NLM abbreviation: Proc Natl Acad Sci U S A
ISSN: 0027-8424
eISSN: 1091-6490
Publisher: National Academy of Sciences; United States
Grant note: GM32036 / NIGMS NIH HHS GM11156 / NIGMS NIH HHS GM35463 / NIGMS NIH HHS
Language: English
Date published: 09/1988
Academic Unit: Obstetrics and Gynecology; Biochemistry and Molecular Biology
Record Identifier: 9984025262002771

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