Genetic knockdown of brain-derived neurotrophic factor in the nervous system attenuates angiotensin II-induced hypertension in mice

Zhongming Zhang; Yijing Zhang; Yan Wang; Shengchen Ding; Chenhui Wang; Li Gao; Alan Johnson; Baojian Xue

doi:10.1177/1470320319834406

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Genetic knockdown of brain-derived neurotrophic factor in the nervous system attenuates angiotensin II-induced hypertension in mice

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Genetic knockdown of brain-derived neurotrophic factor in the nervous system attenuates angiotensin II-induced hypertension in mice

Zhongming Zhang, Yijing Zhang, Yan Wang, Shengchen Ding, Chenhui Wang, Li Gao, Alan Johnson and Baojian Xue

Journal of the renin-angiotensin-aldosterone system, Vol.20(1), pp.1470320319834406-9

01/2019

DOI: 10.1177/1470320319834406

PMCID: PMC6429654

PMID: 30894041

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Abstract

Brain-derived neurotropic factor (BDNF) is expressed throughout the central nervous system and peripheral organs involved in the regulation of blood pressure, but the systemic effects of BDNF in the control of blood pressure are not well elucidated. We utilized loxP flanked BDNF male mice to cross with nestin-Cre female mice to generate nerve system BDNF knockdown mice, nestin-BDNF (+/-), or injected Cre adenovirus into the subfornical organ to create subfornical organ BDNF knockdown mice. Histochemistry was used to verify injection location. Radiotelemetry was employed to determine baseline blood pressure and pressor response to angiotensin II (1000 ng/kg/min). Real-time polymerase chain reaction was used to measure the expression of renin-angiotensin system components in the laminal terminalis and peripheral organs. Nestin-BDNF (+/-) mice had lower renin-angiotensin system expression in the laminal terminalis and peripheral organs including the gonadal fat pad, and a lower basal blood pressure. They exhibited an attenuated hypertensive response and a weak or similar modification of renin-angiotensin system component expression to angiotensin II infusion. Subfornical organ BDNF knockdown was sufficient for the attenuation of angiotensin II-induced hypertension. Central BDNF, especially subfornical organ BDNF is involved in the maintenance of basal blood pressure and in augmentation of hypertensive response to angiotensin II through systemic regulation of the expression of renin-angiotensin system molecules.

Angiotensin II

Animals

Blood Pressure

Brain-Derived Neurotrophic Factor - metabolism

Gene Knockdown Techniques

Hypertension - metabolism

Hypertension - physiopathology

Male

Mice

Nervous System - metabolism

Nestin - metabolism

Renin-Angiotensin System

Subfornical Organ - metabolism

Details

Title: Subtitle: Genetic knockdown of brain-derived neurotrophic factor in the nervous system attenuates angiotensin II-induced hypertension in mice
Creators: Zhongming Zhang - 1 Zhang Zhongjing College of Chinese Medicine, Henan Key Laboratory of Zhang Zhongjing's Formulea for Immunoregulation, Nanyang Institute of Technology, China
Yijing Zhang - 1 Zhang Zhongjing College of Chinese Medicine, Henan Key Laboratory of Zhang Zhongjing's Formulea for Immunoregulation, Nanyang Institute of Technology, China
Yan Wang - 1 Zhang Zhongjing College of Chinese Medicine, Henan Key Laboratory of Zhang Zhongjing's Formulea for Immunoregulation, Nanyang Institute of Technology, China
Shengchen Ding - 1 Zhang Zhongjing College of Chinese Medicine, Henan Key Laboratory of Zhang Zhongjing's Formulea for Immunoregulation, Nanyang Institute of Technology, China
Chenhui Wang - 1 Zhang Zhongjing College of Chinese Medicine, Henan Key Laboratory of Zhang Zhongjing's Formulea for Immunoregulation, Nanyang Institute of Technology, China
Li Gao - 1 Zhang Zhongjing College of Chinese Medicine, Henan Key Laboratory of Zhang Zhongjing's Formulea for Immunoregulation, Nanyang Institute of Technology, China
Alan Johnson - 2 Department of Psychological and Brain Sciences, University of Iowa, USA
Baojian Xue - 2 Department of Psychological and Brain Sciences, University of Iowa, USA
Resource Type: Journal article
Publication Details: Journal of the renin-angiotensin-aldosterone system, Vol.20(1), pp.1470320319834406-9
DOI: 10.1177/1470320319834406
PMID: 30894041
PMCID: PMC6429654
NLM abbreviation: J Renin Angiotensin Aldosterone Syst
ISSN: 1470-3203
eISSN: 1752-8976
Grant note: DOI: 10.13039/501100017278, name: Nanyang Institute of Technology, award: NIT201401015; DOI: 10.13039/501100006407, name: natural science foundation of henan province, award: 162300410203; DOI: 10.13039/100000009, name: Foundation for the National Institutes of Health, award: HL 98207; DOI: 10.13039/100000009, name: Foundation for the National Institutes of Health, award: HL14388
Language: English
Date published: 01/2019
Academic Unit: Psychological and Brain Sciences; Neuroscience and Pharmacology; Neurology (Pediatrics); Health, Sport, and Human Physiology
Record Identifier: 9984213416902771

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