Genetic susceptibility to preeclampsia : Roles of cytosine-to-thymine substitution at nucleotide 677 of the gene for methylenetetrahydrofolate reductase, 68-base pair insertion at nucleotide 844 of the gene for cystathionine β-synthase, and factor V Leiden mutation

Young Ju Kim; Roger A WILLIAMSON; Jeffrey C MURRAY; Janet ANDREWS; Jorie J PIETSCHER; Peter J PERAUD; David C MERRILL

doi:10.1067/mob.2001.110411

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Genetic susceptibility to preeclampsia : Roles of cytosine-to-thymine substitution at nucleotide 677 of the gene for methylenetetrahydrofolate reductase, 68-base pair insertion at nucleotide 844 of the gene for cystathionine β-synthase, and factor V Leiden mutation

Journal article

Peer reviewed

Genetic susceptibility to preeclampsia : Roles of cytosine-to-thymine substitution at nucleotide 677 of the gene for methylenetetrahydrofolate reductase, 68-base pair insertion at nucleotide 844 of the gene for cystathionine β-synthase, and factor V Leiden mutation

Young Ju Kim, Roger A WILLIAMSON, Jeffrey C MURRAY, Janet ANDREWS, Jorie J PIETSCHER, Peter J PERAUD and David C MERRILL

American journal of obstetrics and gynecology, Vol.184(6), pp.1211-1217

2001

DOI: 10.1067/mob.2001.110411

PMID: 11349190

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Abstract

Objective: The purpose of this study was to evaluate the association between preeclampsia and 3 relatively common mutations that are important in the development of vascular disease and thrombosis; these are similar to conditions observed in pregnancies complicated by preeclampsia. Study Design: Deoxyribonucleic acid was extracted from whole blood or cheek swabs of 281 patients with preeclampsia and 360 control subjects (all white). Control subjects consisted of women who had undergone at least 2 term pregnancies unaffected by preeclampsia. Mutation frequencies among patients with preeclampsia and control subjects were compared by standard χ 2 analysis, with P < .05 considered significant. Results: Thirty-three of 281 women with preeclampsia (11.7%) and 22 of 193 women with severe preeclampsia (11.4%) were homozygous for cytosine-to-thymine substitution at nucleotide 677 in the gene for methyltetrahydrofolate reductase (MTHFR), versus 41 of 360 control subjects (11.4%; difference not significant). Forty of 258 women with preeclampsia (15.5%) and 22 of 175 women with severe preeclampsia (12.6%) were heterozygous for the insertion of 68 bases at position 844 in the gene for cystathionine β-synthase (CBS), versus 58 of 332 control subjects (17.5%). Fifteen of 250 women with preeclampsia (6.0%) and 11 of 169 with severe preeclampsia (6.5%) were heterozygous for the Leiden mutation (glycine-to-alanine substitution at nucleotide 1691) in the gene for factor V (F5), versus 12 of 253 control subjects (4.7%; difference not significant). Conclusion: In this white population a missense mutation of MTHFR, an insertion mutation of CBS, and a missense mutation of F5 were not found to be associated with an increased risk for preeclampsia, either independently or in combination.

Gynecology. Andrology. Obstetrics

Biological and medical sciences

Pregnancy. Fetus. Placenta

Medical sciences

Diseases of mother, fetus and pregnancy

Details

Title: Subtitle: Genetic susceptibility to preeclampsia : Roles of cytosine-to-thymine substitution at nucleotide 677 of the gene for methylenetetrahydrofolate reductase, 68-base pair insertion at nucleotide 844 of the gene for cystathionine β-synthase, and factor V Leiden mutation
Creators: Young Ju Kim - Department of Obstetrics and Gynecology, University of Iowa, United States
Roger A WILLIAMSON - Department of Obstetrics and Gynecology, University of Iowa, United States
Jeffrey C MURRAY - Department of Pediatrics, University of Iowa, United States
Janet ANDREWS - Department of Obstetrics and Gynecology, University of Iowa, United States
Jorie J PIETSCHER - Department of Pediatrics, University of Iowa, United States
Peter J PERAUD - Department of Pediatrics, University of Iowa, United States
David C MERRILL - Department of Obstetrics and Gynecology, Wake Forest University, United States
Resource Type: Journal article
Publication Details: American journal of obstetrics and gynecology, Vol.184(6), pp.1211-1217
DOI: 10.1067/mob.2001.110411
PMID: 11349190
NLM abbreviation: Am J Obstet Gynecol
ISSN: 0002-9378
eISSN: 1097-6868
Publisher: Elsevier; Philadelphia, PA
Language: English
Date published: 2001
Academic Unit: Anatomy and Cell Biology; Stead Family Department of Pediatrics; Epidemiology; Pediatric Dentistry; Craniofacial Anomalies Research Center; Obstetrics and Gynecology; Dental Research
Record Identifier: 9984025478502771

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